Abstract:
Tissue microarrays (TMAs), also called tissue chips, contain hundreds to thousands of tissue cores obtained from different tissue donor blocks. By using TMA technology, a molecular marker, such as protein, RNA or DNA, can be simultaneously examined in hundreds of different specimens under the same experimental conditions. A growing number of previous studies have introduced different methods for constructing TMAs. Many authors tried to use various methods to implant more tissue cores in a single recipient block, and most of these methods involved reducing the diameter of the tissue cores and/or the spacing between adjacent tissue cores. However, when creating TMAs, it is difficult to reduce the distance between tissue cores to zero except with extremely expensive automatic TMA arrayers. Here, we introduce a novel method to construct a high-density TMA that does not have spacing between the tissue cores. We also introduce a method for preparing a self-made tissue-arraying instrument. With this method and the tissue-arraying instrument, we successfully created a TMA containing 126 tissue cores that were 2 mm in diameter. H&E staining and immunohistochemical staining were performed on the sections cut from the TMA without any tissue spot loss. This method is easy to operate, and the materials for creating the tissue-arraying instrument are inexpensive and can be purchased anywhere. Therefore, this method can be applied in all laboratories.
摘要:
组织微阵列(TMAs),也称为组织芯片,包含从不同的组织供体块获得的数百到数千个组织核。通过使用TMA技术,分子标记,如蛋白质,RNA或DNA,可以在相同的实验条件下同时检查数百个不同的标本。越来越多的先前研究引入了构建TMA的不同方法。许多作者尝试使用各种方法在单个受体块中植入更多的组织核,并且这些方法中的大多数涉及减小组织芯的直径和/或相邻组织芯之间的间距。然而,创建TMA时,除了极其昂贵的自动TMA阵列之外,很难将组织芯之间的距离减小到零。这里,我们介绍了一种新的方法来构建组织核之间没有间距的高密度TMA.我们还介绍了一种自制组织阵列仪的制备方法。用这种方法和组织排列仪器,我们成功创建了包含126个直径为2mm的组织核的TMA.对从TMA上切下的切片进行H&E染色和免疫组织化学染色,没有任何组织斑点损失。这种方法操作简单,制造组织排列仪器的材料便宜,可以在任何地方购买。因此,这种方法可以应用于所有实验室。