背景:Bothropsmoojeni蛇毒(VBm)具有毒素,可引起明显的组织损伤并加剧炎症反应。大麻是产生富含不饱和脂肪酸的油(CSO)的植物物种。纳米乳液有几个优点,如膜中更好的稳定性和更高的穿透力。因此,这项研究评估了基于这种草药衍生物(NCS)的纳米乳液对Wistar大鼠VBm诱导的炎症的影响。
方法:CSO和NCS进行了物理化学表征。VBm(0.10mg/kg)诱导炎症过程如下:大鼠爪水肿,腹膜炎,大鼠腓肠肌白细胞浸润及肉芽肿组织形成分析。
结果:当NCS进行离心和热应力测试时,未观察到显着变化。没有相分离,密度(0.978±0.01g/cm3)和粘度(0.889±0.15)的变化。液滴直径范围为119.7±065至129.3±0.15nm,多分散指数范围为0.22±0.008至0.23±0.011。结果表明,CSO(200和400mg/kg)和NCS(100mg/kg)处理能够显着减少(p<0.001)水肿和肉芽肿组织的形成。在腹膜炎和白细胞浸润试验中,CSO和NCS组显著减弱(p<0.001)炎性细胞的募集。腓肠肌的组织病理学分析显示由VBm引起的组织损伤减少。
结论:本研究中获得的结果显示CSO的抗炎活性,这可能是由于高UFA含量。纳米尺寸,正如在NCS中掺入CSO所证明的那样,改善了效果,并为获得纳米药物开辟了前景,其中可以在低剂量下使用动力学稳定的植物疗法。
BACKGROUND: Bothrops moojeni snake venom (VBm) has toxins that cause pronounced tissue damage and exacerbated inflammatory reaction. Cannabis sativa L. is a plant species that produces an oil (CSO) rich in unsaturated fatty acids. Nano-emulsions have several advantages, such as better stability and higher penetrating power in membranes. Therefore, this study evaluated the effect of a nano-emulsion based on this herbal derivative (NCS) against VBm-induced inflammation in Wistar rats.
METHODS: The CSO and NCS were submitted to physicochemical characterization. The inflammatory process was induced by the VBm (0.10 mg/kg) as follows: rat paw edema, peritonitis, analysis of leukocyte infiltrate in gastrocnemius muscle of rats and formation of granulomatous tissue.
RESULTS: No significant changes were observed when the NCS was submitted to the centrifugation and thermal stress tests. There was no phase separation, changes in density (0.978 ± 0.01 g/cm3) and viscosity (0.889 ± 0.15). The droplet diameter ranged from 119.7 ± 065 to 129.3 ± 0.15 nm and the polydispersity index ranged from 0.22 ± 0.008 to 0.23 ± 0.011. The results showed that treatments with CSO (200 and 400 mg/kg) and NCS (100 mg/kg) were able to decrease significantly (p < 0.001) the formation of edema and granulomatous tissue. The CSO and NCS groups significantly attenuated (p < 0.001) the recruitment of inflammatory cells in the tests for peritonitis and leukocyte infiltrate. The histopathological analysis of the gastrocnemius muscle showed a reduction in tissue damage caused by VBm.
CONCLUSIONS: The results obtained in this study showed anti-inflammatory activity of the CSO which may be due to a high UFA content. The nanosizing, as evidenced by the incorporation of the CSO in the NCS improved the effect and opens the perspective for the obtainment of a nanomedicine in which a kinetic stable phytotherapic can be used at low doses.