■结核病(TB)仍然是由结核分枝杆菌(Mtb)引起的重大全球卫生紧急情况。流行病学,传输,基因型,突变模式,结核病的临床后果已在世界范围内得到广泛研究,然而,缺乏有关巴基斯坦Mtb流行病学和突变模式的信息,特别是关于耐多药结核病(MDR-TB)的流行。
■本研究旨在使用线探针测定(LPA)研究Mtb的发生率和相关的突变模式。以前的研究报道了rpoB中的高频率突变,inhA,和katG基因,与对利福平(RIF)和异烟肼(INH)的耐药性有关。因此,目前的研究利用LPA来检测rpoB中的突变,katG,和inhA基因来鉴定多药耐药Mtb。
■对大量Mtb分离株的LPA分析,包括241例痰阳性患者的样本,发现34.85%的分离株被鉴定为耐多药结核病,与世界各地不同地区的报告一致。观察到的最普遍的突变是rpoBS531L和inhA启动子C15T,与对RIF和INH的抗性有关,分别。
■本研究强调了GenoTypeMTBDRplus和MTBDRsl分析作为结核病管理的有价值工具的有效性。这些测定能够快速检测对RIF的抗性,INH,Mtb临床分离株中的氟喹诺酮类药物(FQs),超越了基于固体和液体介质的方法的局限性。这些发现有助于我们了解耐多药结核病流行病学,并提供对巴基斯坦Mtb基因谱的见解。这对于有效的结核病控制策略至关重要。
UNASSIGNED: Tuberculosis (TB) remains a significant global health emergency caused by Mycobacterium tuberculosis (Mtb). The epidemiology, transmission, genotypes, mutational patterns, and clinical consequences of TB have been extensively studied worldwide, however, there is a lack of information regarding the epidemiology and mutational patterns of Mtb in Pakistan, specifically concerning the prevalence of multi-drug resistant TB (MDR-TB).
UNASSIGNED: This study aimed to investigate the incidence of Mtb and associated mutational patterns using the line probe assay (LPA). Previous studies have reported a high frequency of mutations in the rpoB, inhA, and katG genes, which are associated with resistance to rifampicin (RIF) and isoniazid (INH). Therefore, the current study utilized LPA to detect mutations in the rpoB, katG, and inhA genes to identify multi-drug resistant Mtb.
UNASSIGNED: LPA analysis of a large pool of Mtb isolates, including samples from 241 sputum-positive patients, revealed that 34.85% of isolates were identified as MDR-TB, consistent with reports from various regions worldwide. The most prevalent mutations observed were rpoB S531L and inhA promoter C15T, which were associated with resistance to RIF and INH, respectively.
UNASSIGNED: This study highlights the effectiveness of GenoType MTBDRplus and MTBDRsl assays as valuable tools for TB management. These assays enable rapid detection of resistance to RIF, INH, and fluoroquinolones (FQs) in Mtb clinical isolates, surpassing the limitations of solid and liquid media-based methods. The findings contribute to our understanding of MDR-TB epidemiology and provide insights into the genetic profiles of Mtb in Pakistan, which are essential for effective TB control strategies.