■结核病(TB),由结核分枝杆菌(Mtb)引起,仍然是一种致命的传染病。印度占全球结核病负担的三分之一。然而,尚未在Telangana(海得拉巴)人群中使用实时聚合酶链反应(RT-PCR)进行研究。因此,本研究通过检测肺结核(PTB)和肺外结核(EPTB),评估了RT-PCR作为一种快速且非侵入性的诊断TB的作用.
■这项基于医院的研究检查了包含组织(n=537)的1670个样本(900EPTB;770PTB),腹膜液(n=420),痰液(n=166),支气管液(n=126),脑脊液(n=145),腹水(n=76),痰脓(n=78),尿液(n=79),和支气管肺泡液(n=43)样品。使用特异性分离试剂盒分离来自样品的DNA并进行RT-PCR。
■在这项研究中,我们纳入了1670名受试者,54.4%为女性,45.6%为男性.收集的样本被归类为流体样本的48.5%,其次是组织(32.2%),痰液(9.9%),尿液(4.7%),和脓液拭子(4.6%)。RT-PCR分析显示4.7%的患者Mtb阳性。我们的结果显示,受影响的患者中有61%是男性,39%是女性。在标本类型中,组织样本的阳性结果比例最高(36.3%).
■结果表明,应在结核病诊断中实施RT-PCR并将其放在首位,以节省时间并促进明确的诊断。强烈建议组织样品通过RTPCR技术筛选Mtb。未来的研究应将该技术扩展到全球人群,并应进行外显子组测序分析以确定结核病风险标志物。
UNASSIGNED: Tuberculosis (TB), caused by Mycobacterium tuberculosis (Mtb), remains a deadly infectious disease. India contributes to one-third of the global TB burden. However, no studies have been carried out in the Telangana (Hyderabad) population using real-time polymerase chain reaction (RT-PCR). Therefore, the current
study evaluated the role of RT-PCR as a rapid and non-invasive test to diagnose TB by testing for pulmonary tuberculosis (PTB) and extrapulmonary tuberculosis (EPTB).
UNASSIGNED: This hospital-based
study examined 1670 samples (900 EPTB; 770 PTB) comprising tissue (n = 537), peritoneal fluid (n = 420), sputum (n = 166), bronchial fluid (n = 126), cerebrospinal fluid (n = 145), ascetic fluid (n = 76), sputum pus (n = 78), urine (n = 79), and bronchoalveolar fluid (n = 43) samples. DNA from samples was separated using specific isolation kits and subjected to RT-PCR.
UNASSIGNED: In this
study, we enrolled 1670 subjects and categorized 54.4% as females and 45.6% as males. The collected samples were categorized as 48.5% of fluid samples, followed by tissue (32.2%), sputum (9.9%), urine (4.7%), and pus-swab (4.6%). RT-PCR analysis revealed that 4.7% patients were positive for Mtb. Our results revealed that 61% of the affected patients were male and 39% were female. Among the specimen types, tissue samples gave the highest proportion of positive results (36.3%).
UNASSIGNED: The results showed that RT-PCR should be implemented and given top priority in TB diagnosis to save time and facilitate a definitive diagnosis. Tissue samples are highly recommended to screen the Mtb through the technique RTPCR. Future studies should extend the technique to the global population and exome sequencing analysis should be performed to identify TB risk markers.