目的:评价部分铒:钇铝石榴石(Er:YAG)激光治疗小鼠模型的效率和机制。
背景:Morphea是一种罕见的自身免疫性疾病,其特征是皮肤中胶原蛋白过度沉积。分数Er:YAG激光治疗是一种很有前途的治疗方法,尽管关于治疗效果和潜在机制的研究有限。
方法:通过皮下注射博来霉素(BLM)建立小鼠角膜模型。总共24只小鼠接受分级Er:YAG激光治疗,每周一次,持续4周。采用的客观测量方法是超声成像测量真皮厚度。主观测量包括根据调整后的局部角膜皮肤评估工具(LoSCAT)进行评分;苏木精和伊红(H&E)染色以评估纤维化的组织学等级;定量形态计量学研究,以通过免疫组织化学确定转化生长因子-β1(TGF-β1)和基质金属蛋白酶-1(MMP1)的表达。
结果:在这项自我对照研究中,分数Er:YAG激光治疗可显着改善硬伤的严重程度,包括较低的临床评分(p<0.01),真皮厚度降低(p<0.001),纤维化组织学分级下降(p<0.001),MMP1增加(p<0.001),TGF-β1表达降低(p<0.01)。
结论:我们发现分数阶Er:YAG激光治疗角膜有良好的临床效果,超声波,和组织病理学疗效,这可能是未来有希望的治疗方法。
OBJECTIVE: To assess the efficiency and the mechanism of fractional erbium:yttrium aluminum garnet (Er:YAG) laser for the treatment of
morphea in mouse model.
BACKGROUND: Morphea is a rare autoimmune disease characterized by excessive collagen deposition in skin. Fractional Er:YAG laser treatment is a promising treatment to improve
morphea, despite limited studies about the therapeutic effect and underlying mechanism.
METHODS: The mouse model of
morphea was established by subcutaneously injecting with bleomycin (BLM). A total of 24 mice received fractional Er:YAG laser treatment once a week for 4 weeks. Objective measurement employed was ultrasonic imaging to measure dermal thickness. Subjective measures included scoring according to the adjusted Localized
morphea Cutaneous Assessment Tool (LoSCAT); hematoxylin and eosin (H&E) staining to evaluate the histological grade of fibrosis; and quantitative morphometric studies to determine the expression of transforming growth factor-β1 (TGF-β1) and matrix metalloproteinase-1 (MMP1) by immunohistochemistry.
RESULTS: In this self-controlled study, fractional Er:YAG laser treatment significantly ameliorate the severity of morphea, including lower clinical score (p < 0.01), decreased dermal thickness (p < 0.001), declined histological grade of fibrosis (p < 0.001), increased MMP1 (p < 0.001), and reduced TGF-β1 (p < 0.01) expression.
CONCLUSIONS: We found that fractional Er:YAG laser treatment of morphea has good clinical, ultrasonic, and histopathologic efficacy, which may be a promising treatment in the future.