椰子油蛋糕（COC），石油开采的副产品，含有高水平的纤维素。这项研究的目的是从腐烂的大丽花中分离出纤维素降解酵母，该酵母可以有效地利用COC作为纤维素酶分泌的唯一碳源。基于筛选,MeyerozymaguillermondiiCBS2030(M.guillermondii)被确定为潜在候选人，在分离的酵母菌株中纤维素分解活性最高，羧甲基纤维素酶(CMCase)活性在第5天达到102.96U/mL。在由GuillermondiiM.降解之前和之后评估COC样品中的纤维素。场发射扫描电子显微镜（FESEM）分析表明，COC结构在处理过程中发生了显着变化，表明有效的水解。改性官能团的傅里叶变换红外光谱（FTIR）表明椰子饼成功解聚。X-射线衍射(XRD)和色差分析确定了GuillermondiiM.COC的有效降解。结果表明，Guillermondii有效地分泌CMCase并降解纤维素，这在COC降解方面具有重要的现实意义。
Coconut oil cake (COC), a byproduct of oil extraction, contains high levels of cellulose. The aim of this study was to isolate a cellulose-degrading yeast from rotten dahlia that can effectively use COC as the only carbon source for cellulase secretion. Based on screening, Meyerozyma guillermondii CBS 2030 (M. guillermondii) was identified as a potential candidate, with the highest cellulolytic activity among the yeast strains isolated, with the carboxymethyl cellulase (CMCase) activity reaching 102.96 U/mL on day 5. The cellulose in COC samples was evaluated before and after degradation by M. guillermondii. Analysis based on field emission scanning electron microscopy (FESEM) revealed that the COC structure was changed significantly during the treatment, indicating effective hydrolysis. Fourier transform infrared spectroscopy (FTIR) of the modified functional groups indicated successful depolymerization of coconut cake. X-ray diffraction (XRD) and analysis of color differences established effective degradation of COC by M. guillermondii. The results demonstrate that M. guillermondii effectively secretes CMCase and degrades cellulose, which has important practical significance in COC degradation.
PDF(Pubmed)