在这项研究中,太子参多糖(PHP)对生长的影响,发展,通过饲喂雏鸡日粮研究雏鸡的肝脏代谢。选取7d龄固始公鸡400只,随机分为4组,标记为A,B,C,和D.A组饲喂基础饮食,B组,C,和D每公斤基础日粮饲喂100、200和400毫克PHP,分别。在14、21、28和35岁时,每组随机抽取5只小鸡样本进行指标检测。结果表明,与A组相比,在14、21和28d时,平均日采食量(ADFI)和饲料重量比(F/G)显着降低(P<0.05),平均日增重(ADG)在21、28d显著增加(P<0.05),总蛋白(TP)水平显着增加,白蛋白(ALB),胰岛素(INS),甲状腺素(T3),生长激素(GH)在14、28d(P<0.05),葡萄糖(GLU)水平显着降低,总胆固醇(TC),胰高血糖素(GC),C组28d时甘油三酯(TG)(P<0.05)。TP水平显著升高,ALB在14、21d(P<0.05),35d时TP水平显著升高(P<0.05),28d时GH水平显著升高(P<0.05),GLU水平显著降低,GC在28d(P<0.05),B组和D组在14、21d时F/G显著降低(P<0.05)。基于以上结果,选择A组和C组28d小鸡的肝脏进行转录组测序。测序结果表明,显著差异表达基因(SDEGs)在生长发育过程中富集,氧化磷酸化,PPAR信号通路和脂质代谢通路。所有这些结果表明,在饮食中添加200mg/kg的PHP促进了生长发育,雏鸡的脂质代谢和能量代谢,抑制炎症和肿瘤的发展,改善肝脏的功能.
为了探索太子参多糖(PHP)作为绿色健康饲料添加剂的可能性,我们评估了PHP对增长的影响,本研究通过饲喂雏鸡日粮对雏鸡发育和肝脏代谢的影响。结果表明,饲粮中添加200mg/kgPHP促进了生长发育,雏鸡脂质代谢和能量代谢,改善肝功能。PHP可能是一种潜在的天然和安全的饲料添加剂应用于家禽生产。
In this study, the effects of Pseudostellaria heterophylla polysaccharide (PHP) on the growth, development, and liver metabolism of chicks were investigated by feeding chicks diets. Four hundred 7-d-old Gushi roosters were selected and randomly divided into four groups, labeled A, B, C, and D. Group A was fed the basal diet, and Groups B, C, and D were fed 100, 200, and 400 mg PHP per kilogram of basal diet, respectively. At 14, 21, 28 and 35 d of age, five chicks were randomly selected from each group to collect samples for index detection. The results showed that compared with Group A, there were significant reduction in average daily feed intake (ADFI) and feed-to-weight ratio (F/G) at 14, 21, and 28 d (P < 0.05), significant increase in average daily gain (ADG) at 21, 28 d (P < 0.05), significantly increased levels of total protein (TP), albumin (ALB), insulin (INS), thyroxine (T3), growth hormone (GH) at 14, 28 d (P < 0.05), significantly decreased levels of glucose (GLU), total cholesterol (TC), glucagon (GC), and triglyceride (TG) at 28 d in Group C (P < 0.05). There were significantly increased levels of TP, ALB at 14, 21 d (P < 0.05), significantly increased level of TP at 35 d (P < 0.05), significantly increased level of GH at 28 d (P < 0.05), significantly decreased levels of GLU, GC at 28 d (P < 0.05), significant reduction in F/G at 14, 21 d in Groups B and D (P < 0.05). Based on the above results, the livers from chicks in Groups A and C at 28 d were selected for transcriptome sequencing. The sequencing results showed that significantly differentially expressed genes (SDEGs) were enriched in growth and development, oxidative phosphorylation, the PPAR signaling pathway and the lipid metabolism pathway. All these results revealed that the addition of 200 mg/kg PHP in the diet promoted the growth and development, lipid metabolism and energy metabolism of chicks, inhibit inflammation and tumor development, and improve the function of the liver.
In order to explore the possibility of Pseudostellaria heterophylla polysaccharide (PHP) as green and healthy feed additive, we evaluated the effects of PHP on the growth, development and liver metabolism of chicks by feeding chicks diets in this study. The results revealed that the addition of 200 mg/kg PHP in the diet promoted the growth and development, lipid metabolism and energy metabolism in chicks and improved liver function. PHP may be a potential natural and safe feed additive applied in poultry production.