自2010年以来,坦布苏病毒(TMUV)在中国高度流行,给家禽业造成重大经济损失。2022年,位于安徽省的一个鹅养殖场发生了疑似TMUV疫情。一株TMUV,从感染的鹅中分离出TMUVHQ-22。使用HQ-22菌株的E基因进行的系统发育分析表明其与簇3的隶属关系,与主要循环簇相比,该簇较少报道。集群2。通过将HQ-22的包膜(E)蛋白与其他典型的TMUV菌株进行比较,在第157个氨基酸位置的突变被鉴定,其中簇3中的缬氨酸(V)变为丙氨酸(A),集群2特有的特征。这些发现强调了在中国传播的TMUV菌株的多样性和复杂性。在我们的实验分析中,将TMUVHQ-22注射到3日龄雏鹅的肌肉中会导致严重的神经系统症状和60%的死亡率。同样,TMUVHQ-22对3周龄ICR小鼠的颅内或鼻内感染导致严重的神经系统症状,死亡率分别为100%或10%.总之,我们的研究分离出了一个TMUV菌株,TMUVHQ-22,来自属于3簇的鹅,在小鹅和ICR小鼠中均表现出明显的致病性。这些结果强调了在中国传播的TMUV的遗传多样性,并将宿主范围扩大到蚊子以外,包括鸭子,鸡,鹅,甚至老鼠。至关重要的是不要低估哺乳动物中TMUV感染的风险,保证我们最大的关注。
Since 2010, the Tembusu virus (TMUV) has been highly prevalent in
China, causing significant economic losses to the poultry industry. In 2022, a suspected outbreak of TMUV occurred at a goose farm located in Anhui Province. A strain of TMUV, TMUV HQ-22, was isolated from the infected geese. Phylogenetic analysis using the E gene of the HQ-22 strain demonstrated its affiliation with cluster 3, a less commonly reported cluster in comparison to the main circulating cluster, cluster 2. Through a comparison of the envelope (E) protein of HQ-22 with other typical TMUV strains, a mutation at the 157th amino acid position was identified, wherein valine (V) in cluster 3 changed to alanine (A), a characteristic that is unique to cluster 2. These findings highlight the diversity and complexity of the TMUV strains circulating in
China. In our experimental analysis, an injection of TMUV HQ-22 into the muscles of 3-day-old goslings resulted in severe neurological symptoms and a mortality rate of 60%. Similarly, the intracranial or intranasal infection of 3-week-old ICR mice with TMUV HQ-22 led to severe neurological symptoms and respective mortality rates of 100% or 10%. In summary, our study isolated a TMUV strain, TMUV HQ-22, from geese that belongs to cluster 3 and exhibits significant pathogenicity in both goslings and ICR mice. These results emphasize the genetic diversity of the TMUV circulating in
China and expand the host range beyond mosquitoes to include ducks, chickens, geese, and even mice. It is crucial to not underestimate the risk of TMUV infection in mammals, warranting our utmost attention.