细菌感染对伤口愈合构成重大障碍，需要开发具有内在抗菌性能的敷料。在这项研究中,据报道，多层伤口敷料（STPU@MTAI2/AM1），包括与抗微生物水凝胶偶联的表面超疏水处理的聚氨酯(STPU)海绵支架。通过应用氟化氧化锌纳米颗粒（F-ZnONPs）在亲水性PU海绵上建立了超疏水保护外层，从而抵抗环境污染和细菌入侵。粘合和抗菌内层是通过N-[2-（甲基丙烯酰氧基）乙基]-N，N,N-三甲基碘化铵和丙烯酰胺，表现出对皮肤表面的有效粘附和针对弹性细菌菌株和生物膜形成的广谱抗微生物作用。STPU@MTAI2/AM1保持透气性和灵活性，确保伤口部位的舒适性和一致性。通过血液相容性和细胞相容性研究证明了多层敷料的生物相容性。多层伤口敷料已证明在处理MRSA感染的伤口时促进伤口愈合的能力。与市售聚氨酯海绵敷料相比,水凝胶层在剥离时没有表现出二次损伤。STPU@MTAI2/AM1处理的伤口到第14天几乎完全愈合，平均伤口面积为12.2%±4.3%，明显低于其他组。此外，STPU@MTAI2/AM1组的CD31表达显著高于其他组,促进伤口中的血管生成,从而有助于伤口愈合。因此,制备的多层伤口敷料为处理感染伤口提供了有希望的治疗候选物。重要性声明：慢性伤口的愈合需要避免生物污染和细菌感染。然而,开发一种既抗生物污染又抗微生物的伤口敷料是一个挑战。开发了具有多功能的多层伤口敷料。它的外层被设计成超疏水的，因此抗生物污染，其内层具有广谱抗菌作用，能抑制生物膜的形成。具有粘合性质的多层伤口敷料可以容易地从伤口表面移除,防止二次损伤的原因。多层伤口敷料已显示出促进MRSA感染的伤口愈合的良好能力，并为MRSA感染的伤口提供了可行的治疗方法。
Bacterial infection poses a significant impediment in wound healing, necessitating the development of dressings with intrinsic antimicrobial properties. In this study, a multilayered wound dressing (STPU@MTAI2/AM1) was reported, comprising a surface-superhydrophobic treated polyurethane (STPU) sponge scaffold coupled with an antimicrobial hydrogel. A superhydrophobic protective outer layer was established on the hydrophilic PU sponge through the application of fluorinated zinc oxide nanoparticles (F-ZnO NPs), thereby resistance to environmental contamination and bacterial invasion. The adhesive and antimicrobial inner layer was an attached hydrogel (MTAI2/AM1) synthesized through the copolymerization of N-[2-(methacryloyloxy)ethyl]-N, N, N-trimethylammonium iodide and acrylamide, exhibits potent adherence to dermal surfaces and broad-spectrum antimicrobial actions against resilient bacterial strains and biofilm formation. STPU@MTAI2/AM1 maintained breathability and flexibility, ensuring comfort and conformity to the wound site. Biocompatibility of the multilayered dressing was demonstrated through hemocompatibility and cytocompatibility studies. The multilayered wound dressing has demonstrated the ability to promote wound healing when addressing MRSA-infected wounds. The hydrogel layer demonstrates no secondary damage when peeled off compared to commercial polyurethane sponge dressing. The STPU@MTAI2/AM1-treated wounds were nearly completely healed by day 14, with an average wound area of 12.2 ± 4.3 %, significantly lower than other groups. Furthermore, the expression of CD31 was significantly higher in the STPU@MTAI2/AM1 group compared to other groups, promoting angiogenesis in the wound and thereby contributing to wound healing. Therefore, the prepared multilayered wound dressing presents a promising therapeutic candidate for the management of infected wounds. STATEMENT OF SIGNIFICANCE: Healing of chronic wounds requires avoidance of biofouling and bacterial infection. However developing a wound dressing which is both anti-biofouling and antimicrobial is a challenge. A multilayered wound dressing with multifunction was developed. Its outer layer was designed to be superhydrophobic and thus anti-biofouling, and its inner layer was broad-spectrum antimicrobial and could inhibit biofilm formation. The multilayered wound dressing with adhesive property could easily be removed from the wound surface preventing the cause of secondary damage. The multilayered wound dressing has demonstrated good abilities to promote MRSA-infected wound healing and presents a viable treatment for MRSA-infected wound.
