背景:隆突性皮肤纤维肉瘤(DFSP)是一种罕见的,真皮低度恶性肿瘤术后复发率高。目前的治疗方法,包括手术,放射治疗,和靶向治疗,有局限性。使用5-氨基乙酰丙酸(5-ALA)的光动力疗法(PDT)是一种有前途的非侵入性方法,但其在DFSP治疗中的疗效仍未得到充分开发。
方法:本研究旨在使用源自复发性DFSP患者的体外模型评估5-ALAPDT的抗肿瘤功效。用不同浓度的5-ALA处理细胞并暴露于红光,然后评估细胞活力,扩散,凋亡,迁移,入侵,血管生成,以及DFSP相关基因和蛋白的表达。
结果:5-ALAPDT以剂量依赖性方式显著降低DFSP细胞活力并诱导细胞凋亡。它还能有效抑制细胞增殖,迁移,和入侵,以及在条件培养基中抑制的血管生成活性。此外,5-ALAPDT下调COL1A1和PDGFRB的表达,DFSP发病的关键基因。
结论:研究结果为5-ALAPDT对DFSP的体外抗肿瘤功效提供了第一个证据,提示其作为DFSP新型治疗方法的潜力。需要进一步的研究来探索5-ALAPDT在预防DFSP复发中的临床效用。
BACKGROUND: Dermatofibrosarcoma Protuberans (DFSP) is a rare, low-grade malignant tumor of the dermis with a high recurrence rate post-surgery. Current treatments, including surgery, radiotherapy, and targeted therapy, have limitations. Photodynamic therapy (PDT) with 5-aminolevulinic acid (5-ALA) is a promising non-invasive approach, but its efficacy in DFSP treatment remains underexplored.
METHODS: This study aimed to evaluate the anti-tumor efficacy of 5-ALA PDT using an in vitro model derived from a recurrent DFSP patient. The cells were treated with varying concentrations of 5-ALA and exposed to red light, followed by assessments of cell viability, proliferation, apoptosis, migration, invasion, angiogenesis, and expression of DFSP-related genes and proteins.
RESULTS: 5-ALA PDT significantly reduced DFSP cell viability in a dose-dependent manner and induced apoptosis. It also effectively inhibited cell proliferation, migration, and invasion, as well as suppressed angiogenic activity in conditioned media. Furthermore, 5-ALA PDT downregulated the expression of COL1A1 and PDGFRB, key genes in DFSP pathogenesis.
CONCLUSIONS: The findings provide the first evidence of 5-ALA PDT\'s in vitro anti-tumor efficacy against DFSP, suggesting its potential as a novel therapeutic approach for DFSP. Further studies are warranted to explore the clinical utility of 5-ALA PDT in preventing DFSP recurrence.