Abstract：
The blood-brain barrier (BBB) is a barrier between the circulatory system and the central nervous system (CNS), contributing to CNS protection and maintaining the brain homeostasis. Establishment of in vitro BBB models that are closer to the microenvironment of the human brain is helpful for evaluating the potential and efficiency of a drug penetrating BBB and thus the clinical application value of the drug. The in vitro BBB models not only provide great convenience for screening new drugs that can access to CNS but also help people to have a deeper study on the mechanism of substances entering and leaving the brain, which makes people have greater opportunities in the treatment of CNS diseases. Up to now, although much effort has been paid to the researches on the in vitro BBB models and many progresses have been achieved, no unified method has been described for establishing a BBB model and there is much work to do and many challenges to be faced with in the future. This review summarizes the research progresses in the establishment, evaluation, and application of in vitro BBB models.
摘要：
血脑屏障（BBB）是循环系统和中枢神经系统（CNS）之间的屏障，有助于中枢神经系统保护和维持大脑稳态。建立更接近人脑微环境的体外BBB模型有助于评估药物穿透BBB的潜力和效率，从而评估该药物的临床应用价值。体外BBB模型不仅为筛选可进入中枢神经系统的新药提供了极大的便利，而且有助于人们对物质进入和离开大脑的机制有更深入的研究。这使得人们在治疗中枢神经系统疾病方面有更多的机会。到目前为止,尽管体外BBB模型的研究已经付出了很大的努力，并取得了许多进展，目前还没有描述建立血脑屏障模型的统一方法，未来还有许多工作要做，面临许多挑战。本文综述了国内外研究进展,评估，和体外BBB模型的应用。
