■甲状腺相关眼病(TAO)被认为是器官特异性自身免疫性疾病。多形核中性粒细胞(PMN)与TAO的发病机理有关。然而,对PMN在TAO发展中的作用知之甚少,TAO中PMN与B细胞和CD4+T细胞之间的关系要小得多。
■本研究旨在探讨PMN的表型特征以及PMN与CD4+T细胞和B细胞亚群在TAO发病机制中的关系。
■收集了135名TAO患者的血常规信息,95例无TAO(GD)患者的Grave病,和116个正常控制(NC),40例TAO患者外周血中PMN的表面标志物表达及CD4+T细胞和B细胞亚群水平,17例GD患者,通过流式细胞术评估45个NC。
■PMN的水平,CD62L+PMN,CD54+PMN,CD4+T细胞,TAO患者的Th17细胞比NC增加,而与NC相比,TAO组的Treg细胞较低。各组间Th1和浆细胞无统计学差别。PMN与Th17细胞呈正相关,但不是Th1Treg,和浆细胞。
■在本研究中,我们发现TAO中PMN和PMN亚群细胞的百分比明显高于NC,PMN与Th17细胞呈正相关。这表明PMN可能参与TAO的免疫发病,并在此过程中调节Th17细胞的反应。
UNASSIGNED: Thyroid-associated ophthalmopathy (TAO) is considered to be an organ-specific autoimmune disease. Polymorphonuclear neutrophils (PMN) have been implicated in the pathogenesis of TAO. However, little is known about the role of PMN in the development of TAO, much less the relationship between PMN with B cells and CD4+T cells in TAO.
UNASSIGNED: This study aims to investigate the phenotypic characteristics of PMN and the relationship between PMN with CD4+T cell and B cell subsets in the pathogenesis of TAO.
UNASSIGNED: Blood routine information was collected from 135 TAO patients, 95 Grave\'s disease without TAO (GD) patients, and 116 normal controls (NC), while surface marker expression of PMN and the level of CD4+T cell and B cell subsets in peripheral blood from 40 TAO patients, 17 GD patients, and 45 NC was assessed by flow cytometry.
UNASSIGNED: The level of PMN, CD62L+PMN, CD54+PMN, CD4+T cells, and Th17 cells displayed an increase in TAO patients than NC, while Treg cells were lower in the TAO group compared to NC. There was no statistical difference in Th1 and plasma cells among the groups. PMN were positively correlated with Th17 cells, but not the Th1, Treg, and plasma cells.
UNASSIGNED: In the present study, we found that the percentage of PMN and PMN subset cells was significantly higher in TAO than in NC, and PMN were positively correlated with Th17 cells. It suggests that PMN may be involved in the immunopathogenesis of TAO and modulate the Th17 cell response during this process.