背景:先前的研究已经证明了跑步机运动(EX)对骨质疏松症的有益作用,高压氧(HBO)对体外成骨细胞和破骨细胞形成的影响。我们研究了HBO以及HBO和EX的组合对去卵巢大鼠骨质疏松症的影响。
方法:将40只3月龄雌性Sprague-Dawley大鼠随机分为5组(n=8):假手术对照组(Control);卵巢切除组;带跑台运动的卵巢切除治疗组;带HBO治疗的卵巢切除组;带HBO治疗的卵巢切除联合跑台运动组。HBO暴露量为203kPa,85-90%O2,90分钟,运动方案为20米·分钟,40分钟·天,5°坡度。两种治疗都是每天一次,一周五天,持续12周,直到大鼠被处死。
结果:所有三种治疗方法(HBO,锻炼,并且两者结合)显着促进了成骨细胞相关基因和氧化代谢相关基因(PGC-1α)的表达。它们还对破骨细胞相关mRNA表达(RANKL)和骨吸收标记物CTX-I具有显着的抑制作用。此外,运动和联合运动-HBO治疗可增加血清超氧化物歧化酶(SOD)和硬化素的表达。没有观察到显著的组间差异。
结论:高压氧,锻炼,和组合改善大鼠骨微结构恶化和卵巢切除术引起的骨丢失,这些抑制作用可能与SOD的增加和PGC-1α的上调有关。
BACKGROUND: Previous studies have demonstrated the beneficial effects of treadmill exercise (EX) on osteoporosis, and of hyperbaric oxygen (HBO) on osteoblast and osteoclast formation in vitro. We investigated the effects of HBO and the combination of HBO and EX on osteoporosis in ovariectomized rats.
METHODS: Forty 3-month-old female Sprague-Dawley rats were randomly divided into 5 groups (n = 8): a sham control group (Control); an ovariectomy group; an ovariectomy with treadmill exercise treatment group; an ovariectomy with HBO treatment group; and an ovariectomy with HBO treatment combined with treadmill exercise group. The HBO exposures were 203 kPa, 85-90% O₂, 90 min and the exercise regimen was 20 m·min⁻¹, 40 min·day¹, 5° slope. Both treatments were administered once daily, five days a week for 12 weeks until the rats were sacrificed.
RESULTS: All three treatments (HBO, exercise, and both combined) significantly promoted the expression of the osteoblast-related gene and oxidative metabolism-related gene (PGC-1α). They also exerted significant inhibitory effects on the osteoclast-related mRNA expression (RANKL) and bone resorption marker CTX-I. Additionally, exercise and the combination exercise-HBO treatment increased serum superoxide dysmutase (SOD) and sclerostin expression. No significant between-group difference was observed.
CONCLUSIONS: Hyperbaric oxygen, exercise, and the combination ameliorated bone microarchitecture deterioration and ovariectomy-induced bone loss in rats, and these inhibitory effects may be associated with the increased SOD and up-regulated PGC-1α.