■为了研究参芪补气散(SQBQP)对平均日增重的影响,血液指标,胃肠道微生物,和小牛的血清代谢产物.
■将总共105头小牛随机分为三组(每组n=35):对照组(C,用基础饮食喂养21天)和两个治疗组(SQBQP-L和SQBQP-H,饲喂基础日粮,补充15和30g/kg的SQBQP),分别为21天。使用LC-MS/MS鉴定SQBQP的活性成分。用ELISA试剂盒和生化试剂盒测定血清消化酶和抗氧化指标,分别。通过液相色谱-质谱/质谱(LC-MS/MS)分析血清差异代谢物,通过16SrDNA测序分析瘤胃液和粪便中的菌群。进一步对SQBQP-H组和C组的胃肠道菌群和血清代谢产物进行Spearman相关性分析。
■SQBQP的主要活性成分主要包括多糖,黄酮类化合物,有机酸。与对照组(C)相比,SQBQP-H(高剂量)和SQBQP-L(低剂量)组的小牛血清淀粉酶(AMS)水平显着增加(P<0.001),脂肪酶含量显著降低(P<0.05)。此外,平均每日收益,T-AOC,SQBQP-H组奶牛纤维素酶含量显著升高(P<0.05)。SQBQP-H组瘤胃菌群中的变形杆菌和丁氏弧菌明显低于C组(P<0.05)。变形杆菌的相对丰度,放线菌,念珠菌_杆菌属,异常球菌_Thermus,蓝细菌,SQBQP-H组明显升高(P<0.05),Tenericte和Oscillibacter的相对丰度显着降低(P<0.05)。血清代谢组学分析显示20种差异代谢物,主要富含氨基酸的生物合成,β-丙氨酸代谢,酪氨酸,和色氨酸生物合成代谢途径(P<0.05)。相关性分析结果表明,瘤胃菌群中的Butyrivibrio和肠道菌群中的镰刀菌属与平均日增重呈极显著正相关,血清生化指标,差异代谢产物(-)-表没食子儿茶素(R>0.58,P<0.05)。
■SQBQP可以通过调节胃肠道菌群和体内代谢过程来促进小牛体重增加并增进健康。
UNASSIGNED: To investigate the effects of Shen Qi Bu Qi Powder (SQBQP) on the average daily gain, blood indexes, gastrointestinal microflora, and serum metabolites of calves.
UNASSIGNED: A total of 105 calves were randomly assigned to three groups (n = 35 per group): the control group (C, fed with a basal diet for 21 days) and two treatment groups (SQBQP-L and SQBQP-H, fed with the basal diet supplemented with 15 and 30 g/kg of SQBQP), respectively for 21 days. The active components of SQBQP were identified using LC-MS/MS. Serum digestive enzymes and antioxidant indices were determined by ELISA kits and biochemical kits, respectively. Serum differential metabolites were analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS), while flora in rumen fluid and fecal were analyzed by 16S rDNA sequencing. Further correlation analysis of gastrointestinal flora and serum metabolites of SQBQP-H and C groups were performed with Spearman\'s correlation.
UNASSIGNED: The principal active components of SQBQP mainly includes polysaccharides, flavonoids, and organic acids. Compared to the control group (C), calves in the SQBQP-H (high dose) and SQBQP-L (low dose) groups showed a significant increase in serum amylase (AMS) levels (P<0.001), while lipase content significantly decreased (P<0.05). Additionally, the average daily gain, T-AOC, and cellulase content of calves in the SQBQP-H group significantly increased (P<0.05). Proteobacteria and Succinivibrio in the rumen flora of the SQBQP-H group was significantly lower than that of the C group (P<0.05). The relative abundance of Proteobacteria, Actinobacteria, Candidatus_Saccharibacteria, Deinococcus_Thermus, Cyanobacteria, and Succinivibrio in the SQBQP-H group was significantly increased (P<0.05), while the relative abundance of Tenericutes and Oscillibacter was significantly decreased (P<0.05). Serum metabolomics analysis revealed 20 differential metabolites, mainly enriched in amino acid biosynthesis, β-alanine metabolism, tyrosine, and tryptophan biosynthesis metabolic pathways (P<0.05). Correlation analysis results showed that Butyrivibrio in rumen flora and Oscillibacter_valericigenes in intestinal flora were significantly positively correlated with average daily gain, serum biochemical indexes, and differential metabolite (-)-Epigallocatechin (R>0.58, P<0.05).
UNASSIGNED: SQBQP can promote calves weight gain and enhance health by modulating gastrointestinal flora and metabolic processes in the body.