由乳酸菌产生的胞外多糖具有多种功能。在本研究中,一个抗氧化多糖部分,即S1-EPS,从乳酸片球菌S1中提取和纯化,研究了其结构及其对脂肪替代品肉碎肉凝胶特性的潜在影响。结果表明,S1-EPS,一种均质多糖,主要由Gal组成,Glc,和Man的摩尔比为7.61:15.25:77.13,分子量为46.975kDa。EPS-S1的主链包含→2,6)-α-D-Manp-(1→,→2)-α-D-Manp-(1→,→3)-α-D-Glcp-(1→和少量→6)-β-D-Manp-(1→。EPS-S1中分支的连接主要由α-D-Manp-(1→连接到糖残基→2,6)-α-D-Manp-(1→O-2或β-D-Galp-(1→连接到糖残基→2,6)-α-D-Manp-(1→O-6。此外,随着S1-EPS的增加,肉末凝胶毛孔减少,表面变得光滑。随着S1-EPS浓度的增加,发现对肉末凝胶的脂质氧化具有显着的抑制作用。总的来说,S1-EPS被发现在低脂肉制品中具有巨大的潜力,抗氧化剂,和功能添加剂。
Exopolysaccharide produced by lactic acid bacteria has various functions. In the present study, one anti-oxidant polysaccharide fraction, namely S1-EPS, was extracted and purified from Pediococcus acidilactici S1, and its structure and its potential effect on the gel properties of fat substitute meat mince were investigated. The results showed that S1-EPS, one of homogeneous polysaccharides, was mainly composed of Gal, Glc, and Man in molar ratio of 7.61: 15.25: 77.13 and molecular weight of 46.975 kDa. The backbone of EPS-S1 contained →2,6)-α-D-Manp-(1→,→2)-α-D-Manp-(1→,→3)-α-D-Glcp-(1 → and a small amount of→6)-β-D-Manp-(1→. The linkages of branches in EPS-S1 were mainly composed of α-D-Manp-(1→ attached to a sugar residue →2,6)-α-D-Manp-(1→O-2 or β-D-Galp-(1→ attached to a sugar residue →2,6)-α-D-Manp-(1→O-6. Furthermore, as S1-EPS increased, the meat minced gel pores decreased, and the surface became smooth. A remarkable inhibitory effect on the lipid oxidation of meat minced gel was found as S1-EPS concentration increased. Overall, S1-EPS was found to have substantial potential in low-fat meat products by serving as a natural, anti-oxidant, and functional additive.