■本研究的目的是通过Granger因果关系分析探讨磷饮食干预后磷代谢网络各因素的贡献。
■在这项研究中,共纳入6名健康男性志愿者.所有参与者依次接受定期,低,和高磷饮食。每个饮食的消耗持续五天,不同饮食之间有5天的冲洗期。在9个时间点(00:00,04:00,08:00,10:00,12:00,14:00,16:00,20:00,24:00)食用每种饮食的第五天收集血液和尿液样本,用于测量血清磷酸盐水平,钙,PTH,FGF23,BALP,α-Klotho,和1,25D和尿磷排泄。使用时间序列数据,通过成对面板Granger因果关系分析,对上述变量在磷网络中的中心性进行了分析。
■参与者的平均年龄为28.5±2.1岁。通过使用格兰杰因果关系分析,我们发现α-Klotho水平与其他变量有最强的联系,并在影响其他变量方面发挥了关键作用。此外,常规磷饮食后,尿磷排泄经常受到磷代谢网络中其他变量的调节。低磷饮食干预后,血清磷酸盐对其他因素影响最大,1,25D水平是主要的结果因素,而尿磷排泄是磷代谢网络中最密切相关的变量。高磷饮食干预后,在格兰杰因果关系分析中,FGF23和1,25D在主动调节和被动调节中发挥了更关键的作用。
■膳食磷摄入量的变化导致参与磷代谢的中心因素发生变化。
BACKGROUND: The purpose of this study was to explore the contribution of each factor of the phosphorus metabolism network following phosphorus diet intervention via Granger causality analysis.
METHODS: In this study, a total of six healthy male volunteers were enrolled. All participants sequentially received regular, low-, and high-phosphorus diets. Consumption of each diet lasted for five days, with a 5-day washout period between different diets. Blood and urinary samples were collected on the fifth day of consumption of each diet at 9 time points (00:00, 04:00, 08:00, 10:00, 12:00, 14:00, 16:00, 20:00, 24:00) for measurements of serum levels of phosphate, calcium, PTH, FGF23, BALP, α-Klotho, and 1,25 D and urinary phosphorus excretion. Granger causality and the centrality of the above variables in the phosphorus network were analyzed by pairwise panel Granger causality analysis using the time-series data.
RESULTS: The mean age of the participants was 28.5 ± 2.1 years. By using Granger causality analysis, we found that the α-Klotho level had the strongest connection with and played a key role in influencing the other variables. In addition, urinary phosphorus excretion was frequently regulated by other variables in the network of phosphorus metabolism following a regular phosphorus diet. After low-phosphorus diet intervention, serum phosphate affected the other factors the most, and the 1,25 D level was the main outcome factor, while urinary phosphorus excretion was the most strongly associated variable in the network of phosphorus metabolism. After high-phosphorus diet intervention, FGF23 and 1,25 D played a more critical role in active regulation and passive regulation in the Granger causality analysis.
CONCLUSIONS: Variations in dietary phosphorus intake led to changes in the central factors involved in phosphorus metabolism.