PDF(Pubmed)
Abstract：
We aimed to determine the onset time of hypophosphatemic rickets and investigate the mechanism of motility impairment through adenosine triphosphate (ATP) production in goslings. Two hundred and sixteen 1-day-old male Jiangnan white geese were randomly divided into 3 groups, with 6 replicates and 12 geese per replicate. Birds were fed on 3 diets: a control diet (nonphytic phosphorus, NPP, 0.38%), a P-deficient diet (PD; NPP, 0.08%), and a high P diet (HP; NPP, 0.80%) for 14 d. Subsequently, all birds were shifted to the control diet for an additional 14 d. The cumulative incidence of lameness increased significantly (P < 0.01) starting on d 4, reaching over 80% on d 7 and 100% on d 12 in the PD group. Drinking and eating frequency decreased from d 4 and d 5, respectively, in the PD group compared to the other groups (most P < 0.01). The PD group exhibited shorter and narrower beaks, higher (worse) curvature scores of the beak and costochondral junctions, swelling caput costae, and dirtier feathers since d 4, in contrast to the control and HP groups (most P < 0.01). The HP had bigger (P < 0.05) beak and sternum sizes than the control groups on d 4 to 11. Leg muscle ATP levels were lower (P < 0.01 or 0.05) on d 4 to 11; in contrast, adenosine diphosphate (d 7-11) was higher in PD compared to the control (P < 0.05). Leg muscle ATP level had positive linear (R2 > 0.40) correlations (r > 0.60) with eating and drinking frequencies on d 7 and 11 (P < 0.01). Bone stiffness, feather cleanliness, and ATP levels recovered (P > 0.05) to the control level, whereas bone size did not recover (P < 0.05) in PD and HP after eating the control diet for 2 wk. The onset time of hypophosphatemic rickets was around 4 d in goslings, and insufficient leg muscle ATP was related to the impaired motility observed in early P-deficient geese.
摘要：
我们旨在确定低磷酸盐血症性of病的发病时间，并研究小牛三磷酸腺苷（ATP）产生的运动性损害的机制。两百十六只1日龄雄性江南白鹅随机分为3组,重复6次，每次重复12只鹅。鸟类以3种饮食为食：对照饮食（非植物磷，NPP,0.38%），缺乏磷的饮食(PD；NPP，0.08%），和高磷饮食(HP；NPP，0.80%)14d。随后，所有禽类均转为对照饮食14d。PD组的跛行累积发生率从第4天开始显着增加（P<0.01），在第7天达到80％以上，在第12天达到100％。饮酒和进食频率分别从d4和d5开始下降，PD组与其他组比拟(最年夜P<0.01)。PD组显示较短和较窄的喙，喙和肋软骨交界处的曲率分数更高（更差），肿胀的羊角，第4天以来，与对照组和HP组相比，羽毛更脏（最P<0.01）。在第4至11天，HP的喙和胸骨大小均大于对照组（P<0.05）。在第4至11天，腿部肌肉ATP水平较低（P<0.01或0.05）；相反，PD中的二磷酸腺苷（d7-11）高于对照组（P<0.05）。在第7天和第11天，腿部肌肉ATP水平与进食和饮酒频率呈正线性（R2>0.40）（r>0.60）（P<0.01）。骨硬度,羽毛清洁度,ATP水平恢复(P>0.05)至对照水平,而服用对照饮食2周后，PD和HP的骨大小未恢复（P<0.05）。雏鹅低磷血症病的发病时间约为4d，腿部肌肉ATP不足与早期P缺乏的鹅运动受损有关。
