昆虫一般气味结合蛋白(GOBPs)在过滤中起着不可替代的作用,绑定,并将宿主气味剂运送到嗅觉受体。Grapholitafunebrana(Treitscheke)(鳞翅目:Tortricidae),一种经济上重要的水果作物害虫,使用水果挥发物作为线索来定位寄主植物。然而,G.funebrana中GOBP的功能仍然未知。三个GOBP基因,即,克隆了GfunGOBP1、GfunGOBP2和GfunGOBP3,并通过实时定量PCR(RT-qPCR)方法检测其在不同组织中的表达谱。通过荧光结合测定法研究了重组GfunGOBP(rGfunGOBP)与各种配体的结合特性。三种GfunGOBP主要在雄性和雌性蛾的触角中表达。所有这三个rGfunGOBP都可以与性信息素结合,同时对这些信息素有不同的亲和力。三种rGfunGOBP还显示了与测试的宿主气味剂的宽范围的配体结合光谱。rGfunGOBP1,rGfunGOBP2和rGfunGOBP3与41种测试气味剂中的34、33和30结合,分别。三个rGfunGOBP对β-月桂烯具有重叠的结合活性,(-)-α-phellandrene,和Ki小于3.0μM的异戊酸乙酯。rGfunGOBP1和rGfunGOBP3可以选择性结合几种杀虫剂,而rGfunGOBP2不能。三个rGfunGOBP具有选择性结合性信息素和宿主气味剂的双重功能。此外,rGfunGOBP1和rGfunGOBP3也可以作为“信号蛋白”并与不同的杀虫剂结合。这项研究有助于阐明G.funebrana嗅觉的潜在分子机制,从而促进开发有效的植物引诱剂或信息素增效剂来控制G.funebrana。
Insect general odorant-binding proteins (GOBPs) play irreplaceable roles in filtering, binding, and transporting host odorants to olfactory receptors. Grapholita funebrana (Treitscheke) (Lepidoptera: Tortricidae), an economically important pest of fruit crops, uses fruit volatiles as cues to locate host plants. However, the functions of GOBPs in G. funebrana are still unknown. Three GOBP genes, namely, GfunGOBP1, GfunGOBP2, and GfunGOBP3, were cloned, and their expression profiles in different tissues were detected by the method of real-time quantitative PCR (RT-qPCR). The binding properties of recombinant GfunGOBPs (rGfunGOBPs) to various ligands were investigated via fluorescence binding assays. The three GfunGOBPs were mainly expressed in the antennae of both male and female moths. All these three rGfunGOBPs could bind to sex pheromones, while having varying affinities toward these pheromones. The three rGfunGOBPs also displayed a wide range of ligand-binding spectrums with tested host odorants. The rGfunGOBP1, rGfunGOBP2, and rGfunGOBP3 bound to 34, 33, and 30 out of the 41 tested odorants, respectively. Three rGfunGOBPs had overlapping binding activities to β-myrcene, (-)-α-phellandrene, and ethyl isovalerate with the Ki less than 3.0 μM. The rGfunGOBP1 and rGfunGOBP3 could selectively bind to several insecticides, whereas rGfunGOBP2 could not. Three rGfunGOBPs had the dual functions of selectively binding to sex pheromones and host odorants. Moreover, the rGfunGOBP1 and rGfunGOBP3 can also serve as \'signal proteins\' and bind to different insecticides. This study contributed to elucidating the potential molecular mechanism of the olfaction for G. funebrana, and thereby promotes the development of effective botanical attractants or pheromone synergists to control G. funebrana.