最近,纳米杀虫剂的使用在控制许多害虫方面显示出显著的功效。然而,纳米农药的作用,尤其是纳米乳液,抑制白蚁,Odontotermesformosanus(Shiraki,1909)(O。formosanus),尚未研究。因此,这项研究旨在生产桉树(EucalyptusglobulusLabill;E-EO)和肉豆蔻(肉豆蔻香精;N-EO)的精油的纳米乳液,以抑制O.formosanus。通过紫外-可见对桉树纳米乳(E-NE)和肉豆蔻纳米乳(N-NE)的分析,动态光散射,zeta电位,透射电子显微镜,扫描电子显微镜,和能量色散光谱。此外,用质谱仪(GC-MS)进行的气相色谱化学分析显示了E-NE和N-NE的主要成分。E-NE的主要化学成分包括D-柠檬烯,桉树脑,1,5-环辛二烯,3,4-二甲基,苯,和1-甲基-3-(1-甲基乙基)-,N-NE的主要成分是环己烷,1-亚甲基-4-(1-甲基乙烯基)-,桉树脑,和L-。阿尔法。-松油醇.死亡率分别为100%和99.53%,分别,用140mg/mL的浓度处理24小时后,与23.43%和43.55%相比,分别,E-EO和N-EO处理。这些结果表明,精油纳米乳液比精油本身更有效。此外,E-NE和N-NE对解毒酶如乙酰胆碱酯酶的影响,羧酸酯酶,酸性和碱性磷酸酶进行了研究,以及总蛋白质浓度,与对照组相比,结果显着增加或减少。此外,显示了暴露于E-NE和N-NE后发现的组织学和形态学改变。总的来说,这项研究的结果清楚地表明,纳米农药配制的纳米乳液可能具有巨大的潜力,用于控制Formosanus的环境安全杀虫剂。
Recently, the use of nanopesticides has shown significant efficacy in the control of many pests. However, the effect of nanopesticides, especially nanoemulsions, on suppressing termites, Odontotermes formosanus (Shiraki, 1909) (O. formosanus), has not been studied yet. Therefore, this study aimed to produce nanoemulsions of the essential oils of eucalyptus (Eucalyptus globulus Labill; E-EO) and nutmeg (Myristica fragrans Houtt; N-EO) to suppress O. formosanus. The analysis of eucalyptus nanoemulsion (E-NE) and nutmeg nanoemulsion (N-NE) was confirmed by using UV-Vis, dynamic light scattering, zeta potential, transmission electron microscopy, scanning electron microscopy, and energy dispersive spectroscopy. In addition, chemical analysis by Gas Chromatography with a mass spectrometer (GC-MS) exhibited the major constituents of E-NE and N-NE. The principal chemical components of E-NE included D-limonene, eucalyptol, 1,5-cyclooctadiene,3,4-dimethyl, benzene, and 1-methyl-3-(1 methylethyl)-, while the main constituents in N-NE were cyclohexane,1-methylene-4-(1 methylethenyl)-, eucalyptol, and L-. alpha. -terpineol. The mortality rates were 100% and 99.53%, respectively, after 24 hours of treatment with a concentration of 140 mg/mL, compared to 23.43% and 43.55%, respectively, from E-EO and N-EO treatment. These results refer to the essential oils\' nanoemulsion as far more effective than the essential oils themselves. Furthermore, the effects of E-NE and N-NE on detoxification enzymes such as acetylcholinesterase, carboxylesterase, acid and alkaline phosphatase were investigated, as well as total protein concentrations, and the results have been found to be significantly increasing or decreasing in comparison with control. Besides, histological and morphological alterations found post exposure to E-NE and N-NE were shown. Overall, the results from this study clearly indicate that the nanopesticide-formulated nanoemulsions may have great potential to be used as novel, environmentally safe insecticides for controlling O. formosanus.