■热杀菌益生菌,作为一种灭活的有益微生物,与活的同行相比,具有延长的保质期和更广泛的适应性。本研究旨在研究热灭活嗜酸乳杆菌(L.嗜酸菌,LA)-一种失活的益生菌对生长性能的影响,消化率,抗氧化状态,兔的免疫力和盲肠微生物群。
■将两百只断奶的Hyla兔随机分为五个相等的组(CON,L200、L400、L600和L800)。在28天的时间里,饲喂基础日粮中添加0、200、400、600和800mg/kg热灭活LA,分别。
■结果表明,L600和L800组的饲料增重比(F/G)显着降低(p<0.05)。此外,L800组粗纤维(CF)和粗蛋白(CP)的表观消化率显着升高(p<0.05)。关于消化酶活性,在L600和L800组中观察到增强的胰蛋白酶和纤维蛋白酶活性(p<0.05)。关于身体抗氧化状态的调节,L800组血清和回肠组织中超氧化物歧化酶(SOD)和总抗氧化能力(T-AOC)水平升高(p<0.05)。在免疫能力调节方面,L600组和L800组血清肿瘤坏死因子-α(TNF-α)水平明显降低(p<0.05),而免疫球蛋白A(IgA)和免疫球蛋白M(IgM)水平较高(p<0.05)。此外,L800组肠粘膜分泌性免疫球蛋白A(SIgA)水平显著升高(p<0.05)。与CON组相比,L800组盲肠中的相肠杆菌和Alistipes的相对丰度显着增加(p<0.05)。根瘤菌与SIgA呈正相关(p<0.05),IgM(p<0.01),和谷胱甘肽过氧化物酶(GSH-Px)(p<0.05),与TNF-α水平呈负相关(p<0.05)。同时,Alistipes与IgA呈正相关(p<0.05),IgM(p<0.05),SGA(p<0.01),GSH-Px(p<0.05),SOD(p<0.05),和T-AOC(p<0.01),与TNF-α呈负相关(p<0.05)。
■总而言之,饲粮添加600mg/kg和800mg/kg的热灭活LA对生长性能有积极影响,营养素消化率,抗氧化状态,兔的免疫能力和盲肠菌群。这突出了在动物营养中利用热灭活益生菌的潜在益处。
UNASSIGNED: Heat-killed probiotics, as a type of inactivated beneficial microorganisms, possess an extended shelf life and broader adaptability compared to their live counterparts. This study aimed to investigate the impact of heat-killed Lactobacillus acidophilus (L. acidophilus, LA) - a deactivated probiotic on the growth performance, digestibility, antioxidant status, immunity and cecal microbiota of rabbits.
UNASSIGNED: Two hundred weaned Hyla rabbits were randomly allocated into five equal groups (CON, L200, L400, L600, and L800). Over a 28-day period, the rabbits were fed basal diets supplemented with 0, 200, 400, 600, and 800 mg/kg of heat-killed LA, respectively.
UNASSIGNED: Results revealed a significant reduction in the feed-to-gain ratio (F/G) in the L600 and L800 groups (p < 0.05). Additionally, the L800 group exhibited significantly higher apparent digestibility of crude fiber (CF) and crude protein (CP) (p < 0.05). Regarding digestive enzyme activities, enhanced trypsin and fibrinase activities were observed in the L600 and L800 groups (p < 0.05). Concerning the regulation of the body\'s antioxidant status, the L800 group demonstrated elevated levels of superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) in both serum and ileal tissue (p < 0.05). In terms of immune capacity modulation, serum tumor necrosis factor-α (TNF-α) levels were significantly lower in the L600 and L800 groups (p < 0.05), while immunoglobulin A (IgA) and immunoglobulin M (IgM) levels were higher (p < 0.05). Additionally, the L800 group exhibited a substantial increase in secretory immunoglobulin A (SIgA) levels in the intestinal mucosa (p < 0.05). In comparison to the CON group, the L800 group exhibited a significant increase in the relative abundance of Phascolarctobacterium and Alistipes in the cecum (p < 0.05). Phascolarctobacterium demonstrated a positive correlation with SIgA (p < 0.05), IgM (p < 0.01), and Glutathione peroxidase (GSH-Px) (p < 0.05), while displaying a negative correlation with TNF-α levels (p < 0.05). Concurrently, Alistipes exhibited positive correlations with IgA (p < 0.05), IgM (p < 0.05), SIgA (p < 0.01), GSH-Px (p < 0.05), SOD (p < 0.05), and T-AOC (p < 0.01), and a negative correlation with TNF-α (p < 0.05).
UNASSIGNED: In conclusion, the dietary incorporation of 600 mg/kg and 800 mg/kg of heat-killed LA positively influenced the growth performance, nutrient digestibility, antioxidant status, immune capacity and cecal microbiota of rabbits. This highlights the potential benefits of utilizing heat-killed probiotics in animal nutrition.