PDF(Pubmed)
Abstract：
This study sought to determine the effects of rosemary leaf powder (RP) on laying performance, egg quality, serum indices, gut barrier function, and cecal microbiota and metabolites of late-phase laying hens. A total of 84 \"Jing Tint 6\" laying hens at 65-week old were randomly divided into 2 groups and fed either a basal diet (CON) or a basal diet supplemented with 0.3% RP. Our study revealed that RP improved the Haugh unit and decreased yolk n-6/n-3 polyunsaturated fatty acid (PUFA) ratio of laying hens, increased serum superoxide dismutase (SOD), jejunal activities of SOD and catalase (CAT), and jejunal zonula occludens-1 (ZO-1) expression, as well as decreased serum tumor necrosis factor-α (TNF-α) level and jejunal TNF-α mRNA expression. Rosemary leaf powder markedly enhanced (P < 0.05) cecal abundances of Rikenellaceae, Rikenellaceae_RC9_gut_group, and Turicibacter, tended to promote (P = 0.076) butyrate concentration, and reduced (P < 0.05) cecal abundances of Erysipelatoclostridiaceae, Sutterellaceae, Fusobacteriaceae, Campylobacteraceae, Sutterella, Campylobacter, and Fusobacterium, which were closely linked with Haugh unit, yolk n-6/n-3 PUFA ratio, serum SOD and TNF-α. In addition, RP altered the metabolic functions of cecal microbiota and enhanced the abundances of butyrate-synthesizing enzymes, including lysine 2,3-aminomutase, β-lysine 5,6-aminomutase, and 3-oxoacid CoA-transferase. Together, 0.3% RP has the potential to enhance egg quality by partially modulating serum antioxidant status, jejunal barrier function, and cecal microbiota structure and metabolites, indicating that RP could be considered a promising feed additive to promote the production performance of late-phase laying hens.
摘要：
本研究旨在确定迷迭香叶粉（RP）对产蛋性能的影响，鸡蛋质量,血清指数，肠屏障功能,以及晚期蛋鸡的盲肠微生物区系和代谢产物。将65周龄的84只“JingTint6”蛋鸡随机分为2组，分别饲喂基础日粮（CON）或添加0.3％RP的基础日粮。我们的研究表明，RP提高了Haugh单位，降低了蛋鸡的蛋黄n-6/n-3多不饱和脂肪酸（PUFA）比率，血清超氧化物歧化酶（SOD）升高，SOD和过氧化氢酶（CAT）的空肠活性，和空肠小带闭塞-1（ZO-1）表达，以及降低血清肿瘤坏死因子-α（TNF-α）水平和空肠TNF-αmRNA表达。迷迭香叶粉显着增强（P<0.05）利肯叶科的盲肠丰度，栗科_RC9_gut_组，和Turicibacter,倾向于促进(P=0.076)丁酸浓度，并降低（P<0.05）赤毒科的盲肠丰度，菊科,梭杆菌科,弯曲杆菌科，Sutterilla,弯曲杆菌,和梭杆菌属，与Haugh单位密切相关，蛋黄n-6/n-3PUFA比，血清SOD和TNF-α。此外,RP改变了盲肠微生物群的代谢功能，增强了丁酸合成酶的丰度，包括赖氨酸2,3-氨基变位酶,β-赖氨酸5,6-氨基变位酶,和3-含氧酸CoA-转移酶。一起,0.3％RP有可能通过部分调节血清抗氧化状态来提高鸡蛋质量，空肠屏障功能，盲肠微生物群结构和代谢产物，表明RP可以被认为是一种有前途的饲料添加剂，以提高后期蛋鸡的生产性能。
