重金属的土壤污染已经成为一个很大的麻烦,导致农业生产的损失,特别是在像巴基斯坦这样的发展中国家,没有合适的渠道来灌溉和提取这些有毒的重金属。本研究旨在通过生长调节剂(α-生育酚150mg/L)和在4°C和50°C下的热压技术来改善重金属离子(Hg-汞)对油菜(BrassicanapusL.)的损害。保持植物的农艺和生理特性。在盆栽实验中,我们总共设计了11种治疗方法。(T0(控制),T1(Hg4ppm),T2(Hg8ppm),T3(Hg4ppm+4°C),T4(Hg4ppm+4°C+生育酚(150米/升)),T5(Hg4ppm+50°C),T6(Hg4ppm+50°C+生育酚(150mg/L)),T7(Hg8ppm+4°C),T8(Hg8ppm+4°C+生育酚(150mg/L)),T9(Hg8ppm+50°C),T10(Hg8ppm+50°C+生育酚(150mg/L)结果表明,叶绿素含量在p<0.05时与生长调节剂和抗氧化酶如过氧化氢酶,过氧化物酶,丙二醛在T5=Hg4ppm+50°C时增加到最大水平(在4ppm氯化汞胁迫下50°C热变形),表明高温引发抗氧化系统以减少光系统损伤。然而,蛋白质,脯氨酸,p<0.05的超氧化物歧化酶和类胡萝卜素,可溶性糖,在8ppm高氯化汞胁迫(T9=Hg8ppm+50°C)下,抗坏血酸过氧化物酶和50°C的高温反应无显著增加(p>0.05),这代表了通过合成渗透物抵抗氧化机制对所选物种的耐受性。此外,在T6=Hg4ppm+50°C+α-生育酚(150mg/L)下,叶面施用α-生育酚和50°C热收缩和4ppm重金属胁迫容易改善%MC(水分含量)的降低,植物活力和发芽能量显著增加。结果表明,通过外源施用α-生育酚和通过合成高水平的脯氨酸和抗氧化活性来维持幼苗生长和发育的热压技术,可以改善仅低浓度(4ppm)重金属胁迫的抑制作用。金属污染土壤。
Soil pollution with heavy metals has grown to be a big hassle, leading to the loss in farming production particularly in developing countries like Pakistan, where no proper channel is present for irrigation and extraction of these toxic heavy metals. The present study aims to ameliorate the damages caused by heavy metal ions (Hg-Mercury) on rapeseed (Brassica napus L.) via a growth regulator (α-tocopherol 150 mg/L) and thermopriming technique at 4 °C and 50 °C to maintain plant agronomical and physiological characteristics. In pot experiments, we designed total of 11 treatments viz.( T0 (control), T1 (Hg4ppm), T2 (Hg8ppm), T3 (Hg4ppm + 4 °C), T4 (Hg4ppm + 4 °C + tocopherol (150 m/L)), T5 (Hg4ppm + 50 °C), T6 (Hg4ppm + 50 °C + tocopherol (150 mg/L)), T7 (Hg8ppm + 4 °C), T8 (Hg8ppm + 4 °C + tocopherol (150 mg/L)), T9 (Hg8ppm + 50 °C), T10 (Hg8ppm + 50 °C + tocopherol (150 mg/L) the results revealed that chlorophyll content at p < 0.05 with growth regulator and antioxidant enzymes such as catalase, peroxidase, and malondialdehyde enhanced up to the maximum level at T5 = Hg4ppm + 50 °C (50 °C thermopriming under 4 ppm mercuric chloride stress), suggesting that high temperature initiate the antioxidant system to reduce photosystem damage. However, protein, proline, superoxide dismutase at p < 0.05, and carotenoid, soluble sugar, and ascorbate peroxidase were increased non-significantly (p > 0.05) 50 °C thermopriming under 8 ppm high mercuric chloride stress (T9 = Hg8ppm + 50 °C) representing the tolerance of selected specie by synthesizing osmolytes to resist oxidation mechanism. Furthermore, reduction in % MC (moisture content) is easily improved with foliar application of α-tocopherol and 50 °C thermopriming and 4 ppm heavy metal stress at T6 = Hg4ppm + 50 °C + α-tocopherol (150 mg/L), with a remarkable increase in plant vigor and germination energy. It has resulted that the inhibitory effect of only lower concentration (4 ppm) of heavy metal stress was ameliorated by exogenous application of α-tocopherol and thermopriming technique by synthesizing high levels of proline and antioxidant activities in maintaining seedling growth and development on heavy metal contaminated soil.