■用大鼠模型研究血平片(XPT)对异常子宫出血(AUB)的保护作用及可能的机制。
■总共选择58只8-9周龄的未交配雌性SD大鼠和25只雄性SPFSD大鼠。按完全随机法选择8只未交配的雌性大鼠作为空白对照组。其他50只大鼠以2:1的雌性/雄性比例交配。交配后的早晨,收集阴道涂片。阴道塞或精子的存在被认为是怀孕的第一天。所有孕鼠于上午8:00灌胃米非司酮8.3mg/kg,妊娠第七天6:00灌胃米索前列醇100μg/kg,诱发不完全性流产,从而建立AUB大鼠模型。将40只大鼠随机分为模型组,低(220毫克/千克),培养基(441mg/kg),高剂量(882mg/kg)XPT,和阳性对照组。阳性组给予宫血宁130mg/kg(GXN)。模型组和空白组给予等量蒸馏水。
■与模型组相比,阳性组和中、高剂量组的出血量减少(P<0.05)。此外,与模型组相比,阳性组和XPT组的孕酮水平显著升高.免疫组化显示XPT显著降低VEGF的表达水平,p-ERK,NF-κB,SAA,MMP-2、MMP-9、TIMP-1、TIMP-2和TIMP-3。WB结果显示XPT显著降低p-ERK的表达水平,MMP-9,NF-κB,MMP-2和VEGF。QRT-PCR结果显示XPT显著降低VEGF的表达水平,NF-κB,SAA,MMP-2、TIMP-1、TIMP-2和TIMP-3(P<0.05)。
■XPT可以通过抑制VEGF-ERK1/2通路中涉及的炎症因子来降低AUB。
UNASSIGNED: To investigate the protective effects against abnormal uterine bleeding (AUB) and possible mechanisms of Xue Ping tablets (XPT) using a rat model.
UNASSIGNED: A total of 58 unmated female and 25 male SPF SD rats aged 8-9 weeks were selected. Eight unmated female rats were selected as the blank control group according to the complete random method. The other 50 rats were mated in a female/male ratio of 2:1. In the morning after mating, vaginal smears were collected. Presence of vaginal plug or sperm was regarded as the first day of pregnancy. All pregnant rats were given 8.3 mg/kg of mifepristone by gavage at 8:00 a.m. and 100 μg/kg misoprostol by gavage at 6:00 p.m. on the seventh day of pregnancy to induce incomplete abortion, thereby establishing a rat model of AUB. Forty rats were randomly divided into model, low- (220 mg/kg), medium- (441 mg/kg), high-dose (882 mg/kg) XPT, and positive control groups. The positive group was given 130 mg/kg Gong Xue Ning (GXN). The model group and the blank group were given an equal amount of distilled water.
UNASSIGNED: Compared with the model group, the volume of bleeding in the positive and middle- and high-dose XPT groups decreased (P < 0.05). Moreover, compared with the model group, the progesterone levels in the positive and XPT groups were significantly increased. Immunohistochemistry showed that XPT significantly decreased the expression levels of VEGF, p-ERK, NF-κB, SAA, MMP-2, MMP-9, TIMP-1, TIMP-2 and TIMP-3. WB results showed that XPT significantly decreased the expression levels of p-ERK, MMP-9, NF-κB, MMP-2 and VEGF. QRT-PCR results showed that XPT significantly decreased the expression levels of VEGF, NF-κB, SAA, MMP-2, TIMP-1, TIMP-2 and TIMP-3 (P < 0.05).
UNASSIGNED: XPT could reduce AUB by inhibiting the inflammatory factors involved in the VEGF-ERK1/2 pathway.