如何在COVID-19之后解决公共卫生优先事项正成为一项关键任务。为此,我们对六种主要病原体进行了废水监测,即,SARS-CoV-2诺如病毒,轮状病毒,甲型流感病毒(IAV),肠道病毒和呼吸道合胞病毒(RSV),2023年1月至4月在南昌市。在1日进行了Metaviromic测序,第四,Seven,9th,第12周和第14周,以揭示qPCR未涵盖的病毒病原体的动态。每周还对诺如病毒GI和GII的保守区以及轮状病毒和编码RSV非结构蛋白的区域进行扩增子测序。结果表明,在2023年1月SARS-CoV-2污水浓度快速下降后,诺如病毒激增,轮状病毒,IAV和RSV在6号开始,Seven,第8周和第11周,分别。诺如病毒污水浓度的动态,轮状病毒,IAV和RSV与上述传染病的淡季复发一致。值得注意的是,诺如病毒GI的峰值污水浓度,GII,轮状病毒,6号发现了IAV和RSV,3rd,Seven,第七和第八周,分别。星状病毒也在第7周后重新流行,正如元病毒数据所揭示的,这表明废水监测与元病毒数据一起为揭示传染病复发的模式提供了一个重要的预警工具。
How to address public health priorities after COVID-19 is becoming a critical task. To this end, we conducted wastewater surveillance for six leading pathogens, namely, SARS-CoV-2, norovirus, rotavirus, influenza A virus (IAV), enteroviruses and respiratory syncytial virus (RSV), in Nanchang city from January to April 2023. Metaviromic sequencing was conducted at the 1st, 4th, 7th, 9th, 12th and 14th weeks to reveal the dynamics of viral pathogens that were not covered by qPCR. Amplicon sequencing of the conserved region of norovirus GI and GII and the rotavirus and region encoding nonstructural protein of RSV was also conducted weekly. The results showed that after a rapid decrease in SARS-CoV-2 sewage concentrations occurred in January 2023, surges of norovirus, rotavirus, IAV and RSV started at the 6th, 7th, 8th and 11th weeks, respectively. The dynamics of the sewage concentrations of norovirus, rotavirus, IAV and RSV were consistent with the off-season resurgence of the above infectious diseases. Notably, peak sewage concentrations of norovirus GI, GII, rotavirus, IAV and RSV were found at the 6th, 3rd, 7th, 7th and 8th weeks, respectively. Astroviruses also resurge after the 7th week, as revealed by metaviromic data, suggesting that wastewater surveillance together with metaviromic data provides an essential early warning tool for revealing patterns of infectious disease resurgence.