由于近年来牧场的退化和放牧范围的严格限制,玉东黑山羊铜(Cu)缺乏症一直在发生,主要表现为消瘦,贫血,食欲不振和缺乏精神。为探讨玉东黑山羊缺铜的主要原因,40只黑山羊(1岁,本实验选择25.11±0.52kg);其中,20只来自实验牧场的铜缺乏的玉东黑山羊作为实验组,以对照牧场的20只健康玉洞黑山羊为对照组。在实验前,土壤中的矿物质含量,牧草,血,并测定两组黑山羊的肝脏,在正式的实验中,血液血液学,生物化学,抗氧化剂,并对血液流变学参数进行了分析。还进行了黑山羊缺铜的治疗实验。这项研究表明,土壤中的硒(Se)水平,牧草,血,实验组肝脏明显低于对照组(p<0.01)。牧草中的硫(S)含量明显高于对照组(p<0.01)。实验组血液和肝脏中的Cu含量明显低于对照组(p<0.01),S含量明显高于对照组(p<0.01)。实验组的血液血液学受到影响,血红蛋白的减少证明了这一点,血细胞比容值,平均红细胞体积和平均红细胞血红蛋白。实验组黑山羊的免疫力和抗氧化能力均有不同程度的损害,铜蓝蛋白显著减少,免疫球蛋白M,免疫球蛋白G,谷胱甘肽过氧化物酶,和超氧化物歧化酶,丙二醛的大量增加。此外,实验组显示出血液粘度的降低,这可以通过高剪切粘度的升高来证明,低剪切粘度,红细胞刚性指数,红细胞聚集指数,和红细胞变形指数,和血浆粘度的降低。在治疗实验中,对10只铜缺乏的黑山羊口服硫酸铜溶液。所有缺铜山羊都被治愈了,他们体内的铜含量反弹。总之,低硒土壤导致牧草中S含量增加,和以高S饲草为食的玉东黑山羊导致铜吸收减少,这导致了二次铜缺乏。
Due to the degradation of pasture and strict restrictions on grazing ranges in recent years, copper (Cu) deficiency in Yudong black goats has been occurring, mainly manifested as emaciation, anemia, loss of appetite and lack of spirit. To explore the main causes of Cu deficiency in Yudong black goats, 40 black goats (1 year old, 25.11 ± 0.52 kg) were selected for this experiment; among them, 20 Yudong black goats with Cu deficiency from the experimental pasture were used as the experimental group, and 20 healthy Yudong black goats from the control pasture were used as the control group. In the pre-experiment, the mineral contents of the soil, forage, blood, and liver of black goats in both groups were determined, and in formal experiments, blood hematological, biochemical, antioxidant, and hemorheological parameters were analyzed. An experiment on the treatment of Cu deficiency in black goats was also conducted. This study showed that selenium (Se) levels in the soil, forage, blood, and liver from the experimental group were significantly lower than those from the control group (p < 0.01). The content of sulfur (S) in the forage was considerably higher than that of the control group (p < 0.01). The contents of Cu in the blood and liver from the experimental group were significantly lower than that from the control group (p < 0.01), and the content of S was considerably higher than that from the control group (p < 0.01). The blood hematology of the experimental group was affected, as evidenced by a decrease in hemoglobin, hematocrit value, mean corpuscular volume and mean corpuscular hemoglobin. The immunity and antioxidant capacity of black goats in the experiment group were impaired to varying degrees, with significant decreases in ceruloplasmin, immunoglobulin M, immunoglobulin G, glutathione peroxidase, and superoxide dismutase, and substantial increases in malondialdehyde. In addition, the experimental group showed a decrease in blood viscosity as evidenced by the rise in high shear viscosity, low shear viscosity, erythrocyte rigidity index, erythrocyte aggregation index, and erythrocyte deformation index, and a decrease in plasma viscosity. In the treatment experiment, oral administration of copper sulfate solution was carried out on 10 black goats with Cu deficiency. All the Cu deficiency goats were cured, and the Cu content in their bodies rebounded. In summary, low Se soil caused an increase in S content in the forage, and Yudong black goats feeding on high S forage resulted in a decrease in Cu absorption, which led to a secondary Cu deficiency.