失眠通常与抑郁症并存,但是潜在的神经元回路机制仍然难以捉摸。最近,我们报道了GABA能腹侧苍白球(VP)神经元控制与动机相关的觉醒。然而,VP神经元的其他亚型是否以及如何调节唤醒和情绪在很大程度上是未知的。这里,我们报道了谷氨酸能VP(VPVglut2)神经元控制清醒和抑郁样行为。生理学上,在小鼠的NREM睡眠-觉醒过渡和抑郁/焦虑样行为期间,VPVglut2神经元的钙活性均增加.功能上,VPVglut2神经元的激活足以增加觉醒和诱导焦虑/抑郁样行为,而抑制减弱了两者。电路的解剖表明,VPVglut2神经元向下丘脑外侧和外侧hu的分离投射促进了唤醒和抑郁样行为,分别。我们的结果表明,VP神经元的亚型通过分离的投影负责觉醒和情绪,并可能为失眠和抑郁症患者的干预提供新的思路。
Insomnia is often comorbid with depression, but the underlying neuronal circuit mechanism remains elusive. Recently, we reported that GABAergic ventral pallidum (VP) neurons control wakefulness associated with motivation. However, whether and how other subtypes of VP neurons regulate arousal and emotion are largely unknown. Here, we report glutamatergic VP (VPVglut2) neurons control wakefulness and depressive-like behaviors. Physiologically, the calcium activity of VPVglut2 neurons was increased during both NREM sleep-to-wake transitions and depressive/anxiety-like behaviors in mice. Functionally, activation of VPVglut2 neurons was sufficient to increase wakefulness and induce anxiety/depressive-like behaviors, whereas inhibition attenuated both. Dissection of the circuit revealed that separated projections of VPVglut2 neurons to the lateral hypothalamus and lateral habenula promote arousal and depressive-like behaviors, respectively. Our results demonstrate a subtype of VP neurons is responsible for wakefulness and emotion through separated projections, and may provide new lines for the intervention of insomnia and depression in patients.