矮竹Sasaargenteostriata(Regel)E.G.在先前的研究中,加缪被认为是金属植物修复的潜在植物。然而,铅(Pb)解毒的机制尚未描述。本研究的目的是探索植物组织中铅解毒的差异策略或机制。在这方面,四种水培铅处理,包括0(对照),进行了300、600和900mgL-1的亚细胞区室化检查,Pb积累/物种和抗氧化剂辅助螯合。我们的发现表明,鞭根系统对Pb的保留是其解毒机制之一,以避免伤害芽。此外,细胞壁保留是鞭子的主要解毒策略,新的根,旧根和新/旧茎,而液泡区隔是新/旧叶子。有趣的是,四种低迁移率/毒性Pb物种(即,FNaCl,FHAC,FHCl和FR)分布在根中,鞭子和茎,而叶片中存在两种高迁移率/毒性Pb物种(FE和FW)。将Pb转化为低毒/迁移是根中的Pb解毒策略,鞭子和茎,但不在叶子中。此外,新/旧根和叶可以通过合成非蛋白质巯基来减轻Pb的伤害,谷胱甘肽和植物螯合素.其中,植物螯合素在新/旧根的解毒中起主导作用,而谷胱甘肽在新/旧叶子中。本研究为矮竹组织从生理到细胞水平的不同铅解毒策略提供了第一个全面的证据。支持该植物可以在受铅污染的介质中进行植物修复。
Dwarf bamboo Sasa argenteostriata (Regel) E.G. Camus is considered as potential plants for metal phytoremediation in previous filed observations. However, the mechanisms of lead (Pb) detoxification has not been described. The objective of this study was to explore the difference strategies or mechanisms of Pb detoxification in plant tissues. In this regard, four Pb treatments with hydroponics including 0 (control), 300, 600, and 900 mg L-1 were conducted to examine subcellular compartmentalization, Pb accumulation/species and antioxidant-assisted chelation. Our findings showed the retention of Pb by the whip-root system is one of its detoxification mechanisms to avoid damage the shoots. In addition, the cell wall retention is the dominant detoxification strategy of whips, new roots, old roots and new/old stems, while vacuolar compartmentalization is for new/old leaves. Interestingly, four low-mobility/-toxicity Pb species (i.e., FNaCl, FHAc, FHCl and FR) are distributed in roots, whips and stems, while two high-mobility/-toxicity Pb species (FE and FW) in leaves. The conversion of Pb to low-toxicity/-migration is a Pb-detoxification strategy in roots, whips and stems but not in leaves. Besides, the new/old roots and leaves can alleviate Pb damage through the synthesis of non-protein thiol, glutathione and phytochelatins. Among these, phytochelatins play a leading role in the detoxification in new/old roots, while glutathione is in new/old leaves. This study provides the first comprehensive evidence regarding the different strategies for Pb detoxification in dwarf bamboo tissues from physiological to cellular level, supporting that this plant could be rehabilitated for phytoremediation in Pb-contaminated media.