噬菌体展示技术已成为生物学研究的重要研究工具,从根本上改变传统的单克隆抗体制备工艺,并已广泛应用于抗原抗体库的建立,药物设计,疫苗研究,病原体检测,基因治疗,抗原表位研究,和细胞信号转导研究。噬菌体展示是技术发展的强大平台。使用噬菌体展示技术,单链片段变量(scFv)可以筛选,取代传统抗体大尺寸的缺点。噬菌体展示单链抗体文库具有显著的生物学意义。这里我们描述了抗体的类型,包括嵌合抗体,双特异性抗体,和scFvs。此外,我们描述了噬菌体展示系统,噬菌体展示单链抗体文库,通过噬菌体文库筛选特异性抗体和噬菌体文库的应用。
Phage display technology has become an important research tool in biological research, fundamentally changing the traditional monoclonal antibody preparation process, and has been widely used in the establishment of antigen-antibody libraries, drug design, vaccine research, pathogen detection, gene therapy, antigenic epitope research, and cellular signal transduction research.The phage display is a powerful platform for technology development. Using phage display technology, single chain fragment variable (scFv) can be screened, replacing the disadvantage of the large size of traditional antibodies. Phage display single chain antibody libraries have significant biological implications. Here we describe the types of antibodies, including chimeric antibodies, bispecific antibodies, and scFvs. In addition, we describe the phage display system, phage display single chain antibody libraries, screening of specific antibodies by phage libraries and the application of phage libraries.