Abstract：
Lung cancer is a dangerous disease that is lacking in an ideal therapy. Here, we evaluated the anti-lung cancer effect in nude mice of a fully human single-chain antibody (scFv) against the associated antigen 7 transmembrane receptor (Ts7TMR), which is also called G protein-coupled receptor, between A549 cells and Trichinella spiralis (T. spiralis). Our data showed that anti-Ts7TMR scFv could inhibit lung cancer growth in a dose-dependent manner, with a tumour inhibition rate of 59.1%. HE staining did not reveal any obvious tissue damage. Mechanistically, immunohistochemical staining revealed that the scFv down-regulated the expression of PCNA and VEGF in tumour tissues. Overall, this study found that anti-Ts7TMR scFv could inhibit A549 lung cancer growth by suppressing cell proliferation and angiogenesis, which may provide a new strategy for treating lung cancer.
摘要：
肺癌是一种危险的疾病，缺乏理想的治疗方法。这里,我们评估了针对相关抗原7跨膜受体（Ts7TMR）的全人单链抗体（scFv）在裸鼠中的抗肺癌作用，也称为G蛋白偶联受体，在A549细胞和旋毛虫之间（T。spiralis).我们的数据表明，抗Ts7TMRscFv可以以剂量依赖的方式抑制肺癌的生长，肿瘤抑制率为59.1%。HE染色未发现明显的组织损伤。机械上,免疫组织化学染色显示scFv下调肿瘤组织中PCNA和VEGF的表达。总的来说,这项研究发现，抗Ts7TMRscFv可以通过抑制细胞增殖和血管生成来抑制A549肺癌的生长，这可能为肺癌的治疗提供新的策略。
