未经授权:尖孢镰刀菌是人参栽培中常见的致病性真菌。已报道病原体和拮抗真菌都能诱导植物抗性反应,从而促进次生代谢产物的积累。本实验的目的是比较两种真菌之一的优势,为了筛选出更有效的激发者。补充了真菌激发子诱导植物抗性反应的机制。
UNASSIGNED:进行梯度稀释和硬脑膜培养以筛选菌株。通过形态学和18srDNA鉴定测试菌株。不同浓度(0、50、100、200、400mg/L)对青霉的影响。测试了YJM-2013和尖孢酵母对鲜重和人参皂苷积累的影响。信号分子转导,研究了转录因子和功能基因的表达,以研究真菌诱导子的诱导机制。
未经鉴定:尖孢酵母的拮抗真菌被鉴定为青霉菌。YJM-2013,降低了根系生物量。用青霉处理的人参不定根的总皂苷含量达到最大值(48.95±0.97mg/g)。YJM-2013,200mg/L,比对照高2.59倍,其中原人参二醇型人参皂苷(PPD)增加了4.57倍。此外,青霉。YJM-2013激活防御信号分子,人参皂苷合成中PgWRKY1、2、3、5、7、9和功能基因的表达上调。
UNASSIGNED:与病原真菌尖孢酵母相比,拮抗真菌青霉。YJM-2013更有利于人参不定根中人参皂苷的积累。青霉。YJM-2013通过增强信号分子的产生促进人参皂苷的积累,激活转录因子和功能基因的表达。
UNASSIGNED: Fusarium oxysporum is a common pathogenic fungus in ginseng cultivation. Both pathogens and antagonistic fungi have been reported to induce plant resistance responses, thereby promoting the accumulation of secondary metabolites. The purpose of this experiment is to compare the advantages of one of the two fungi, in order to screen out more effective elicitors. The mechanism of fungal elicitor-induced plant resistance response is supplemented.
UNASSIGNED: A gradient dilution and the dural culture were carried out to screen strains. The test strain was identified by morphology and 18 s rDNA. The effect of different concentrations (0, 50, 100, 200, 400 mg/L) of Penicillium sp. YJM-2013 and F. oxysporum on fresh weight and ginsenosides accumulation were tested. Signal molecules transduction, expression of transcription factors and functional genes were investigated to study the induction mechanism of fungal elicitors.
UNASSIGNED: Antagonistic fungi of F. oxysporum was identified as Penicillium sp. YJM-2013, which reduced root biomass. The total ginsenosides content of Panax ginseng adventitious roots reached the maximum (48.95 ± 0.97 mg/g) treated with Penicillium sp. YJM-2013 at 200 mg/L, higher than control by 2.59-fold, in which protopanoxadiol-type ginsenosides (PPD) were increased by 4.57 times. Moreover, Penicillium sp. YJM-2013 activated defense signaling molecules, up-regulated the expression of PgWRKY 1, 2, 3, 5, 7, 9 and functional genes in ginsenosides synthesis.
UNASSIGNED: Compared with the pathogenic fungi F. oxysporum, antagonistic fungi Penicillium sp. YJM-2013 was more conducive to the accumulation of ginsenosides in P. ginseng adventitious roots. Penicillium sp. YJM-2013 promoted the accumulation of ginsenosides by intensifying the generation of signal molecules, activating the expression of transcription factors and functional genes.