在海葵毒液中发现的肽毒素具有多种特性,使其成为药理学领域的重要研究课题,神经科学和生物技术。本研究采用高通量测序技术对触角的毒液成分进行系统分析,column,和海葵的肠系膜细丝,揭示海葵毒素在不同组织中的多样性和复杂性。共鉴定了1049份转录本,并将其分为60个家庭,其中91.0%是蛋白质,9.0%是肽。在那1049份成绩单中,从触手中鉴定出416、291和307个推定的蛋白质和肽前体,column,和肠系膜细丝,而在合并数据集时确定了428。在这些假定的毒素序列中,在所有三个组织中检测到42,包括33种蛋白质和9种肽,大多数肽是ShKT结构域,β-防御素,和Kunitz型.此外,本研究应用生物信息学方法来预测家庭分类,3D结构,以及这些代表性肽的功能注释,以及肽之间的进化关系,为下一步肽药理活性研究奠定基础。
Peptide toxins found in sea anemones venom have diverse properties that make them important research subjects in the fields of pharmacology, neuroscience and biotechnology. This study used high-throughput sequencing technology to systematically analyze the venom components of the tentacles, column, and mesenterial filaments of sea anemone Heteractis crispa, revealing the diversity and complexity of sea anemone toxins in different tissues. A total of 1049 transcripts were identified and categorized into 60 families, of which 91.0% were proteins and 9.0% were peptides. Of those 1049 transcripts, 416, 291, and 307 putative proteins and peptide precursors were identified from tentacles, column, and mesenterial filaments respectively, while 428 were identified when the datasets were combined. Of these putative toxin sequences, 42 were detected in all three tissues, including 33 proteins and 9 peptides, with the majority of peptides being ShKT domain, β-defensin, and Kunitz-type. In addition, this study applied bioinformatics approaches to predict the family classification, 3D structures, and functional annotation of these representative peptides, as well as the evolutionary relationships between peptides, laying the foundation for the next step of peptide pharmacological activity research.