目的:本研究旨在介绍一例暴露于海葵喷出的污水后,短暂的角膜损伤,Anthopleurauchidai,并通过实验证实组织培养物中存在来自A.uchidai的有毒物质。
方法:我们回顾了一名51岁男子的临床过程,他抱怨海葵刺痛后左眼视力下降,A.乌基代。评估了A.uchidai流出物在永生化人角膜内皮细胞(HCEnC-21T)和人角膜上皮细胞中的毒性。
结果:观察到角膜水肿,他的最佳矫正视力是0.2。角膜内皮细胞密度下降至1435个细胞/mm2。尽管他的角膜水肿和视力在局部滴注类固醇和5%NaCl后恢复,损伤后3年角膜内皮细胞密度未恢复。体外研究揭示了海葵的分馏流出物,通过尺寸排阻色谱法,含有对HCEnC-21T有毒的物质,具有细胞质肿胀和核错位。
结论:有必要小心沿海海葵的流出物,眼科医生应该意识到海葵会导致角膜内皮功能障碍。
OBJECTIVE: This study aimed to present a
case of transient corneal damage after exposure to the effluent squirting from a sea anemone, Anthopleura uchidai, and to experimentally confirm the presence of toxic substances from an A. uchidai in the tissue culture.
METHODS: We reviewed the clinical course of a 51-year-old man who complained of decreased vision in his left eye after the stinging of a sea anemone, A. uchidai. The toxicity of the effluents from an A. uchidai in immortalized human corneal endothelial cells (HCEnC-21T) and human corneal epithelial cells in vitro were evaluated.
RESULTS: Corneal edema was observed, and his best-corrected visual acuity was 0.2. Corneal endothelial cell density decreased to 1435 cells/mm2. Although his corneal edema and visual acuity recovered after topical instillation with a topical steroid and 5% NaCl, corneal endothelial cell density did not recover for 3 years after the injury. The in vitro study revealed fractioned effluence from the sea anemone, by size-exclusion chromatography, containing a substance toxic to HCEnC-21T with cytoplasmic swelling and nuclear dislocation.
CONCLUSIONS: It is necessary to be cautious of effluents from sea anemones along the coast, and ophthalmologists should be aware that sea anemones can cause corneal endothelial dysfunction.