背景:要比较成对的盒子7(PAX7)的数量-阳性,肌源性分化1(MyoD1)阳性,和神经细胞粘附分子-1(NCAM)-阳性卫星细胞在人原代与继发性下斜肌过度活动(IOOA)。
方法:这项前瞻性观察性研究纳入了2020年1月至2020年11月在天津眼科医院小儿眼科和斜视行下斜肌肌切除术的患者。处理肌肉标本。免疫组织化学和免疫荧光用于定量下斜肌纤维直径和PAX7阳性,NCAM-阳性,和MyoD1阳性卫星细胞。
结果:纳入了38例下斜肌过度活动患者:18例原发性IOOA,20例继发性IOOA。继发性IOOA组的参与者明显年轻于原发性IOOA组(2.8±1.2vs.9.0±3.2年,P<0.001)。两组之间的肌纤维直径没有显着差异(13.5±1.4vs.13.8±0.7µm,P=0.530),但PAX7pos(3.3±2.8vs.1.8±0.6,P<0.001),NCAMpos(3.6±1.5vs.1.7±0.2,P<0.001),和MyoD1pos(4.8±1.9vs.2.7±0.5,P<0.001)原发性IOOA的细胞计数高于继发性IOOA。
结论:PAX7pos,原发性IOOA组的每肌纤维MyoD-1pos和NCAMpos细胞计数几乎比继发性IOOA组高2倍。
BACKGROUND: The aim of the study was to compare the number of paired box 7 (PAX7)-positive, myogenic differentiation 1 (MyoD1)-positive, and neural cell adhesion molecule-1 (NCAM)-positive satellite cells in human primary versus secondary inferior oblique muscle overaction (IOOA).
METHODS: This prospective observational study enrolled patients who underwent inferior oblique muscle myectomy at the Department of Pediatric Ophthalmology and Strabismus in Tianjin Eye Hospital between January 2020 and November 2020. The muscle specimens were processed. Immunohistochemistry and immunofluorescence were used to quantify inferior oblique muscle fiber diameter and PAX7-positive, NCAM-positive, and MyoD1-positive satellite cells.
RESULTS: Thirty-eight patients with inferior oblique overaction were enrolled: 18 with primary IOOA and 20 with secondary IOOA. The participants were significantly younger in the secondary IOOA group than in the primary IOOA group (2.8 ± 1.2 vs. 9.0 ± 3.2 years, p < 0.001). The muscle fiber diameter between the two groups was not significantly different (13.5 ± 1.4 vs. 13.8 ± 0.7 µm, p = 0.530), but PAX7+ (3.3 ± 2.8 vs. 1.8 ± 0.6, p < 0.001), NCAM+ (3.6 ± 1.5 vs. 1.7 ± 0.2, p < 0.001), and MyoD1+ (4.8 ± 1.9 vs. 2.7 ± 0.5, p < 0.001) cell counts were higher in primary IOOA than in secondary IOOA.
CONCLUSIONS: PAX7pos, MyoD-1pos, and NCAMpos cell counts per laminar nucleus were almost 2-fold higher in the primary IOOA group than in the secondary IOOA group.