Abstract：
Astragalus polysaccharide (APS) possesses extensive biological activities, pharmacological effects, and anti-fatigue function. MiR-133a is a specifically expressed miRNA in skeletal muscle that participates in the regulation of myoblast proliferation and differentiation. However, little is known about the role of APS in the development of sheep skeletal muscle. In this study, we aimed to investigate the underlying mechanism of APS and miR-133a on the differentiation of sheep skeletal muscle satellite cells (SMSCs) and the regulatory relationship between APS and miR-133a. The results suggested that APS plays a positive regulatory role in the proliferation and differentiation of sheep SMSCs. Moreover, miR-133a significantly promotes SMSC differentiation and the activity of the MAPK/ERK signaling pathway. Importantly, we found that APS function requires the mediation of miR-133a in the differentiation of sheep SMSCs. Taken together, our results indicate that APS accelerates SMSC differentiation by regulating miR-133a via the MAPK/ERK signaling pathway in sheep.
摘要：
黄芪多糖(APS)具有广泛的生物活性,药理作用，和抗疲劳功能。MiR-133a是骨骼肌中特异性表达的miRNA,参与调控成肌细胞的增殖和分化。然而,人们对APS在绵羊骨骼肌发育中的作用知之甚少。在这项研究中,我们旨在探讨APS和miR-133a对绵羊骨骼肌卫星细胞（SMSCs）分化的潜在机制以及APS和miR-133a之间的调控关系。提示APS对绵羊SMSCs的增殖和分化具有正向调节作用。此外，miR-133a显著促进SMSC分化和MAPK/ERK信号通路的活性。重要的是,我们发现APS功能在绵羊SMSCs分化过程中需要miR-133a的介导。一起来看,我们的结果表明,APS通过MAPK/ERK信号通路调节miR-133a加速绵羊SMSC分化.
