光动力疗法(PDT)在癌症治疗中引起了极大的关注,而其治疗效果受到不令人满意的光敏剂和缺氧肿瘤微环境(TME)的限制。为了解决这些问题,我们已经开发了过氧化氢酶负载的锰-卟啉框架(CAT@MnPFs)用于癌细胞的催化辅助PDT。通过将Mn2离子和PpIX组装成MnPFs并随后加载过氧化氢酶来构建CAT@MnPFs。在650nm的光照射下,CAT@MnPFs结构中的卟啉(原卟啉IX)可以将氧(O2)转化为单线态氧(1O2),显示光动力效果。重要的是,负载的过氧化氢酶可以在600s内将过氧化氢(H2O2)分解为O2,O2水平大幅升高(13.22mgL-1),从而促进通过PDT产生1O2。因此,CAT@MnPFs结合650nm的光可以有效地消融癌细胞,由于过氧化氢酶辅助的氧释放PDT,显示出较高的治疗效果。同时,与CAT@MnPFs孵育后,在正常和红细胞中可以发现不明显的损伤。因此,获得的CAT@MnPFs整合了光敏剂和过氧化氢酶用于放氧PDT的优势,这可以为治疗缺氧细胞提供一些见解。
Photodynamic therapy (PDT) is attracting great attention for cancer treatments, while its therapeutic efficacy is limited by unsatisfactory photosensitizers and hypoxic tumor microenvironment (TME). To address these problems, we have developed catalase-loaded manganese-
porphyrin frameworks (CAT@MnPFs) for catalytically-assisted PDT of cancer cells. CAT@MnPFs were constructed by the assembly of Mn2+ ions and PpIX into MnPFs and the subsequent loading of catalase. Under 650 nm light irradiation, the
porphyrin (Protoporphyrin IX) within the structure of CAT@MnPFs can convert oxygen (O2) into singlet oxygen (1O2), showing the photodynamic effect. Importantly, the loaded catalase can decompose hydrogen peroxide (H2O2) into O2 with a huge elevation of O2 level (13.22 mg L-1) in 600 s, thus promoting 1O2 generation via PDT. As a result, CAT@MnPFs combined with 650 nm light can effectively ablate cancer cells due to the catalase-assisted oxygen-evolving PDT, showing a high therapeutic efficacy. Meanwhile, after the incubation with CAT@MnPFs, unobvious damage can be found in normal and red blood cells. Thus, the obtained CAT@MnPFs integrate the advantage of photosensitizers and catalase for oxygen-evolving PDT, which can provide some insight for treating hypoxic cells.