生物膜形成可能极大地促进细菌中的抗生素抗性,并且因此细菌生物膜的有效去除迫切需要解决。这里,我们报道,补充非抑制浓度的N-乙酰-L-半胱氨酸(NAC),一种常见的活性氧(ROS)清除剂,可以显着降低成熟的铜绿假单胞菌生物膜的生物量(通过结晶紫测定法和激光扫描共聚焦显微镜证实)。处理15天后,1mMNAC将进化的PAO1中的作弊者(ΔlasR突变体)频率提高到89.4±1.5%。通过NAC清除ROS引起铜绿假单胞菌生物膜的崩溃,但它没有改变群体感应调节的基因表达(例如,hcnC和cioAB)和氰化氢生产。公共好蛋白酶的补充有助于生物膜生物量的恢复,表明破坏警务在生物膜抑制中的作用。此外,7种典型的ROS清除剂(例如,超氧化物歧化酶,过氧化氢酶和过氧化物酶,等。)也有效地抑制了成熟的铜绿假单胞菌生物膜。这项研究表明,清除ROS可以通过监控破坏作为复杂水环境中的靶向生物膜控制策略来促进铜绿假单胞菌生物膜的选择性控制。
Biofilm formation is likely to contribute greatly to antibiotic resistance in bacteria and therefore the efficient removal of bacterial biofilms needs addressing urgently. Here, we reported that the supplement of non-inhibitory concentration of N-acetyl-L-cysteine (NAC), a common reactive oxygen species (ROS) scavenger, can significantly reduce the biomass of mature Pseudomonas aeruginosa biofilms (corroborated by crystal violet assay and laser scanning confocal microscopy). 1 mM NAC increased the cheater (ΔlasR mutant) frequency to 89.4 ± 1.5% in the evolved PAO1 after the 15-day treatment. Scavenging of ROS by NAC induced the collapse of P. aeruginosa biofilms, but it did not alter quorum sensing-regulated genes expression (e.g., hcnC and cioAB) and hydrogen cyanide production. The replenishment of public good protease contributed to the recovery of biofilm biomass, indicating the role of disrupting
policing in biofilm inhibition. Furthermore, 7 typical ROS scavengers (e.g., superoxide dismutase, catalase and peroxidase, etc.) also effectively inhibited mature P. aeruginosa biofilms. This study demonstrates that scavenging of ROS can promote the selective control of P. aeruginosa biofilms through
policing disruption as a targeted biofilm control strategy in complex water environments.