接种丛枝菌根能促进植物生长,但关于丛枝菌根真菌与植物组合共生效应差异的具体研究尚不深入。因此,本研究选择紫花苜蓿,BromusinermisLeyss,和高羊茅.,我国退化土地恢复常用的三种AMF分别接种,探讨不同AMF接种方式对不同植物生长性能和养分吸收的影响,为菌根与植物结合的研发提供科学依据。我们建立了四种接种Entrophosporaetunicata(EE)的治疗方法,真菌(FM),根瘤菌内(RI),和非接种。主要研究结果如下:三种AMF与三种草地植物形成了良好的共生关系,RI和FM对株高的接种效应更显著,生物量,和tiller编号。与C相比,紫花苜蓿的地上生物量,BromusinermisLeyss,和高羊茅.接种AMF后增加101.30-174.29%,51.67-74.14%,和110.67-174.67%。AMF接种增强了植物对N的吸收,P,K,植物P和K含量与植物生物量显著相关。对三种植物的PLS-PM分析均表明,AMF接种增加了植物的养分吸收,然后通过增加植物高度和根分ill来增加地上生物量和地下生物量。本研究表明,RI是一种更适合与草地退化恢复草种结合的AMF,并提出了AMF-植物共生增加产量的潜在机制。
Arbuscular mycorrhizal inoculation can promote plant growth, but specific research on the difference in the symbiosis effect of arbuscular mycorrhizal fungi and plant combination is not yet in-depth. Therefore, this study selected Medicago sativa L., Bromus inermis Leyss, and Festuca arundinacea Schreb., which were commonly used for restoring degraded land in
China to inoculate with three AMF separately, to explore the effects of different AMF inoculation on the growth performance and nutrient absorption of different plants and to provide a scientific basis for the research and development of the combination of mycorrhiza and plants. We set up four treatments with inoculation Entrophospora etunicata (EE), Funneliformis mosseae (FM), Rhizophagus intraradices (RI), and non-inoculation. The main research findings are as follows: the three AMF formed a good symbiotic relationship with the three grassland plants, with RI and FM having more significant inoculation effects on plant height, biomass, and tiller number. Compared with C, the aboveground biomass of Medicago sativa L., Bromus inermis Leyss, and Festuca arundinacea Schreb. inoculated with AMF increased by 101.30-174.29%, 51.67-74.14%, and 110.67-174.67%. AMF inoculation enhanced the plant uptake of N, P, and K, and plant P and K contents were significantly correlated with plant biomass. PLS-PM analyses of three plants all showed that AMF inoculation increased plant nutrient uptake and then increased aboveground biomass and underground biomass by increasing plant height and root tillering. This study showed that RI was a more suitable AMF for combination with grassland degradation restoration grass species and proposed the potential mechanism of AMF-plant symbiosis to increase yield.