背景:光热敏雄性不育(PTMS),指的是由光周期和温度变化引发的雄性不育,是小麦两系杂交系统中的关键要素。在两系杂交小麦中,雄性不育系的安全生产和高效繁殖的发展至关重要。在稳定的光周期条件下,PTMS主要由小麦的高温或低温引起,但日温差(DTD)对PTMS系育性转换的影响尚未见报道。这里,使用三个BS型PTMS品系,包括BS108,BS138和BS366,以及对照小麦品种J411,使用差异播种试验分析了育性与DTD之间的相关性,光-温控实验,和转录组测序。
结果:差异播种试验表明,3个PTMS品系安全种子生产的最佳播种时间为10月5日至25日在邓州,中国。在12h12°C的条件下,PTMS品系受DTD的影响很大,并且在15°C的温差下表现出完全的雄性不育。此外,在不同的温差条件下,使用RNA测序共获得20,677个差异表达基因(DEGs).此外,通过加权基因共表达网络分析(WGCNA)和KEGG富集分析,确定的DEGs与“淀粉和蔗糖代谢”密切相关,“苯丙素生物合成”,\“MAPK信号通路-植物\”,“类黄酮生物合成”,和\"角质,以及木纹和蜡的生物合成\“。qRT-PCR分析显示,在温差为15℃时,KEGG通路相关核心基因的表达水平显著降低。我们构建了一个转录组介导的温度差异网络,影响雄性不育。
结论:这些发现为温度差与雄性不育之间的相关性提供了重要的理论见解,为识别和选择更安全和有效的PTMS线路提供指导。
BACKGROUND: Photo-thermo-sensitive male sterility (PTMS), which refers to the male sterility triggered by variations in photoperiod and temperature, is a crucial element in the wheat two-line hybrid system. The development of safe production and efficient propagation for male sterile lines holds utmost importance in two-line hybrid wheat. Under the stable photoperiod condition, PTMS is mainly induced by high or low temperatures in wheat, but the effect of daily temperature difference (DTD) on the fertility conversion of PTMS lines has not been reported. Here, three BS type PTMS lines including BS108, BS138, and BS366, as well as a control wheat variety J411 were used to analyze the correlation between fertility and DTD using differentially sowing tests, photo-thermo-control experiments, and transcriptome sequencing.
RESULTS: The differentially sowing tests suggested that the optimal sowing time for safe seed production of the three PTMS lines was from October 5th to 25th in Dengzhou,
China. Under the condition of 12 h 12 °C, the PTMS lines were greatly affected by DTD and exhibited complete male sterility at a temperature difference of 15 °C. Furthermore, under different temperature difference conditions, a total of 20,677 differentially expressed genes (DEGs) were obtained using RNA sequencing. Moreover, through weighted gene co-expression network analysis (WGCNA) and KEGG enrichment analysis, the identified DEGs had a close association with \"starch and sucrose metabolism\", \"phenylpropanoid biosynthesis\", \"MAPK signaling pathway-plant\", \"flavonoid biosynthesis\", and \"cutin, and suberine and wax biosynthesis\". qRT-PCR analysis showed the expression levels of core genes related to KEGG pathways significantly decreased at a temperature difference of 15 ° C. Finally, we constructed a transcriptome mediated network of temperature difference affecting male sterility.
CONCLUSIONS: The findings provide important theoretical insights into the correlation between temperature difference and male sterility, providing guidance for the identification and selection of more secure and effective PTMS lines.