Abstract：
Cytoplasmic male sterility (CMS) is a very important factor to produce hybrid seeds, and the restoration of fertility involves the expression of many fertility-related genes. Our previous study showed that the expression of CaPIPLC5 was significantly up-regulated in pepper restorer accessions and minimally expressed in sterile accessions, speculating that CaPIPLC5 is related to the restoration of fertility. In this study, we further validated the function of CaPIPLC5 in the restoration of fertility. The results showed that CaPIPLC5 was specifically expressed in the anthers of the restorer accessions with the subcellular localization in the cytoplasm. Furthermore, the expression of CaPIPLC5 was significantly higher in restorer lines and restorer combinations than that in CMS lines and their maintainer lines. Silencing CaPIPLC5 led to the number of pollen decreased, pollen grains wrinkled, and the ratio of pollen germination reduced. In addition, the joint analysis of Yeast One-Hybrid (Y1H) and Dual-Luciferase (dual-LUC) assays suggested that transcription factors such as CaARF5, CabZIP24 and CaMYB-like1, interacted with the promoter regions of CaPIPLC5, which regulated the expression of CaPIPLC5. The present results provide new insights into the study of CaPIPLC5 involved in the restoration of fertility in pepper.
摘要：
细胞质雄性不育(CMS)是产生杂交种子的重要因素,生育力的恢复涉及许多与生育力相关的基因的表达。我们先前的研究表明，CaPIPLC5的表达在辣椒恢复材料中显著上调，在不育材料中表达最低。推测CaPIPLC5与生育能力的恢复有关。在这项研究中,我们进一步验证了CaPIPLC5在恢复生育力中的功能。结果表明，CaPIPLC5在恢复系的花药中特异性表达，并在细胞质中具有亚细胞定位。此外，恢复系和恢复系组合中CaPIPLC5的表达明显高于CMS系及其维持系。沉默CaPIPLC5导致花粉数量减少，花粉粒起皱，花粉萌发率降低。此外,酵母单杂交（Y1H）和双荧光素酶（双LUC）分析的联合分析表明，诸如CaARF5，CabZIP24和CaMYB-like1之类的转录因子与CaPIPLC5的启动子区相互作用，从而调节了CaPIPLC5的表达。本研究结果为CaPIPLC5参与辣椒生育力恢复的研究提供了新的见解。
