食用菌在蘑菇出苗阶段具有一定的光敏性,但是,很少有相关的研究关于石竹对不同光质的反应。L.dastes被种植在具有不同光质的生长室中,分别,白光(CK),单色红光(R),单色蓝光(B),红色和蓝色混合光(RB),以及远红光和蓝光(FrB)的混合物。通过分析L.dastes的生长特性,研究了L.dastes的光敏性,营养质量,暴露于不同光处理的蘑菇中的胞外酶以及光感受器基因。结果表明,R导致菌丝变性,乳膏中真菌皮肤失活和原始形成失败。刀柄长度,刀柄直径,暴露于RB的绒头直径和子实体的重量分别显着增加了8.0、28.7、18.3和58.2%,与对照组相比(p<0.05)。B显著降低刀柄长度和子实体重量,分别下降8.5%和20.2%,与对照组相比(p<0.05)。相对于对照,在用B和FrB处理的L.decastespileus中检测到增加的颜色指标和加深的模拟颜色。同时,在暴露于B和FrB的蘑菇中,WC-1,WC-2和Cry-DASH等蓝色感光基因的表达水平显着上调(p<0.05)。此外,用RB处理的菌毛中粗蛋白和粗多糖的含量分别为,分别,与对照组相比增加了26.5%和9.4%,而stipes的人数增加了5.3%和58.8%,分别。同时,纤维素酶等胞外酶的活性,半纤维素酶,漆酶,锰过氧化物酶,木质素过氧化物酶和淀粉酶在受到RB的蘑菇中显著上调(p<0.05),这可能会促进培养材料的降解。总的来说,在用RB处理的L.desaste中检测到最大的体积和重量以及最高的营养素含量。该研究为工业化生产中的光环境调控提供了理论依据。
Edible fungi has certain photo-sensitivity during the mushroom emergence stage, but there has been few relevant studies on the responses of Lyophyllum decastes to different light quality. L. decastes were planted in growth chambers with different light qualities that were, respectively, white light (CK), monochromatic red light (R), monochromatic blue light (B), mixed red and blue light (RB), and the mixture of far-red and blue light (FrB). The photo-sensitivity of L. decastes was investigated by analyzing the growth characteristics, nutritional quality, extracellular enzymes as well as the light
photoreceptor genes in mushroom exposed to different light treatments. The results showed that R led to mycelium degeneration, fungal skin inactivation and failure of primordial formation in L. decastes. The stipe length, stipe diameter, pileus diameter and the weight of fruiting bodies exposed to RB significantly increased by 8.0, 28.7, 18.3, and 58.2% respectively, compared to the control (p < 0.05). B significantly decreased the stipe length and the weight of fruiting body, with a decrease of 8.5 and 20.2% respectively, compared to the control (p < 0.05). Increased color indicators and deepened simulated color were detected in L. decastes pileus treated with B and FrB in relative to the control. Meanwhile, the expression levels of blue
photoreceptor genes such as WC-1, WC-2 and Cry-DASH were significantly up-regulated in mushroom exposed to B and FrB (p < 0.05). Additionally, the contents of crude protein and crude polysaccharide in pileus treated with RB were, respectively, increased by 26.5 and 9.4% compared to the control, while those in stipes increased by 5.3 and 58.8%, respectively. Meanwhile, the activities of extracellular enzyme such as cellulase, hemicellulase, laccase, manganese peroxidase, lignin peroxidase and amylase were significant up-regulated in mushroom subjected to RB (p < 0.05), which may promote the degradation of the culture materials. On the whole, the largest volume and weight as well as the highest contents of nutrients were all detected in L. decastes treated with RB. The study provided a theoretical basis for the regulation of light environment in the industrial production of high quality L. decastes.