生长激素缺乏症(GHD)和特发性身材矮小(ISS)是最常见的身材矮小(SS)类型,但是对它们的发病机制知之甚少,对青少年SS的研究知之甚少。在这项研究中,基于核磁共振(NMR)的代谢组学分析结合最小绝对收缩和选择算子(LASSO)进行,以识别不同类型的SS(包括94个青春期前GHD(PAG)的生物标志物,61青春期前国际空间站(PAI),43名青少年GHD(ADG),和19个青少年ISS(ADI)),受试者工作特征曲线(ROC)进一步用于评估潜在生物标志物的预测能力。结果表明,十一,九,15种代谢物被鉴定为PAG的潜在生物标志物,PAI,ADG,和ADI与相应的对照相比,分别。青春期前SS的代谢通路紊乱主要为碳水化合物代谢和脂质代谢,而氨基酸代谢紊乱在青少年SS中发挥了重要作用。天冬氨酸的组合,乙醇胺,磷酸胆碱,从PAG中筛选出三甲胺以鉴定PAI,还有丙氨酸,组氨酸,异丁酸,甲醇,磷酸胆碱对ADI和ADC具有较高的分类精度。青春期前和青少年中GHD和ISS之间代谢特征的差异将有助于发展身材矮小的个性化临床治疗。
Growth hormone deficiency (GHD) and idiopathic short stature (ISS) are the most common types of short stature (SS), but little is known about their pathogenesis, and even less is known about the study of adolescent SS. In this study, nuclear magnetic resonance (NMR)-based metabolomic analysis combined with least absolute shrinkage and selection operator (LASSO) were performed to identify the biomarkers of different types of SS (including 94 preadolescent GHD (PAG), 61 preadolescent ISS (PAI), 43 adolescent GHD (ADG), and 19 adolescent ISS (ADI)), and the receiver operating characteristic curve (ROC) was further used to evaluate the predictive power of potential biomarkers. The results showed that fourteen, eleven, nine, and fifteen metabolites were identified as the potential biomarkers of PAG, PAI, ADG, and ADI compared with their corresponding controls, respectively. The disturbed metabolic pathways in preadolescent SS were mainly carbohydrate metabolism and lipid metabolism, while disorders of amino acid metabolism played an important role in adolescent SS. The combination of aspartate, ethanolamine, phosphocholine, and trimethylamine was screened out to identify PAI from PAG, and alanine, histidine, isobutyrate, methanol, and phosphocholine gave a high classification accuracy for ADI and ADC. The differences in metabolic characteristics between GHD and ISS in preadolescents and adolescents will contribute to the development of individualized clinical treatments in short stature.