目的:本研究旨在比较有出血和无出血的扁桃体切除术后患者的扁桃体菌群。并研究扁桃体菌群在扁桃体切除术后出血(PTH)发展中的潜在作用。
方法:收集来自PTH患者的19个扁桃体组织和来自对照组患者的21个组织。宏基因组测序用于比较PTH和对照组中的微生物群。Alpha多样性指数用于比较两组之间微生物群的丰富度和均匀度。使用PCoA和NMDS分析来评估β多样性。进行LDA分析以鉴定显著丰富的属。
结果:在PTH和对照组患者之间没有发现α多样性指数的显着差异。扁桃体微生物群中的优势细菌是嗜血杆菌,链球菌,和梭杆菌.PCoA和NMDS分析显示,PTH和对照组患者之间的β多样性存在显着差异。PTH患者的奈瑟氏球菌的相对丰度明显较高,Capnocytophaga,还有Veillonella.通过LDA分析,Capnocytophaga也被鉴定为非常丰富的属。
结论:这项研究表明,PTH和对照组患者的扁桃体微生物群存在差异。结果表明,奈瑟菌,Capnocytophaga,Veillonella可能与PTH的发生有关。这些发现为扁桃体微生物群在PTH发展中的潜在作用提供了新的见解,并可能有助于开发预防和治疗这种潜在危及生命的并发症的新策略。
OBJECTIVE: This study aimed to compare the tonsillar microbiota between post tonsillectomy patients with bleeding and without bleeding, and to investigate the potential role of tonsillar microbiota in the development of post-tonsillectomy hemorrhage (PTH).
METHODS: Nineteen tonsillar tissues from PTH patients and 21 tissues from control patients were collected. Metagenomic sequencing was used to compare the microbiota in PTH and control groups. Alpha diversity indices were used to compare the richness and evenness of the microbiota between the two groups. PCoA and NMDS analyses were used to evaluate beta diversity. LDA analysis was conducted to identify significantly abundant genera.
RESULTS: No significant difference in alpha diversity indices was found between PTH and control patients. The dominant bacteria in the tonsillar microbiota were Haemophilus, Streptococcus, and Fusobacterium. PCoA and NMDS analyses showed significant differences in beta diversity between PTH and control patients. PTH patients had a significantly higher relative abundance of Neisseria, Capnocytophaga, and Veillonella. Capnocytophaga was also identified as a significantly abundant genus by LDA analysis.
CONCLUSIONS: This study demonstrates that there is a difference in the tonsillar microbiota between PTH and control patients. The results suggest that Neisseria, Capnocytophaga, and Veillonella may be associated with the development of PTH. These findings provide new insights into the potential role of the tonsillar microbiota in the development of PTH, and may help to develop new strategies for preventing and treating this potentially life-threatening complication.