过度暴露于光线是一个全球性问题。人工光污染已被证明会破坏人体的自然昼夜节律。为了研究光对新陈代谢的影响,我们研究了在白天或夜间长期暴露于红色或蓝色光的Sprague-Dawley大鼠。实验组中的大鼠在白天或夜间暴露于延长的光照4小时,以模拟过度光照的影响。引人注目的是,我们发现系统代谢改变仅在白天由蓝光引起.此外,我们对脑脊液进行了代谢组学分析,血清,心,肝脏,脾,脾肾上腺,小脑,垂体,前列腺,精子,大鼠下丘脑和肾脏的对照和白天的蓝光暴露。在脑脊液中观察到代谢物的变化,血清,大鼠的下丘脑和肾脏在白天暴露于蓝光。在包括丙酮酸代谢的大鼠中观察到的代谢改变,谷胱甘肽代谢同型半胱氨酸降解,磷脂酰乙醇胺生物合成,和磷脂生物合成,表现出与特定生理过程中固有的模式相似的模式,特别是神经发育,细胞损伤,氧化应激,和自噬途径。我们的研究提供了对白天暴露于蓝光的大鼠的组织特异性代谢变化的见解,并可能有助于解释光发病的潜在机制。
Excessive exposure to light is a global issue. Artificial light pollution has been shown to disrupt the body\'s natural circadian rhythm. To investigate the impacts of light on metabolism, we studied Sprague-Dawley rats chronically exposed to red or blue light during daytime or nighttime. Rats in the experimental group were exposed to extended light for 4 hours during daytime or nighttime to simulate the effects of excessive light usage. Strikingly, we found systemic metabolic alterations only induced by blue light during daytime. Furthermore, we conducted metabolomic analyses of the cerebrospinal fluid, serum, heart, liver, spleen, adrenal, cerebellum, pituitary, prostate, spermatophore, hypothalamus and kidney from rats in the control and blue light exposure during daytime. Significant changes in metabolites have been observed in cerebrospinal fluid, serum, hypothalamus and kidney of rats exposed to blue light during daytime. Metabolic alterations observed in rats encompassing pyruvate metabolism, glutathione metabolism homocysteine degradation, phosphatidylethanolamine biosynthesis, and phospholipid biosynthesis, exhibit analogous patterns to those inherent in specific physiological processes, notably neurodevelopment, cellular injury, oxidative stress, and autophagic pathways. Our study provides insights into tissue-specific metabolic changes in rats exposed to blue light during the daytime and may help explain potential mechanisms of photopathogenesis.