作为食物过敏的交叉反应性碳水化合物决定因素的提议，聚糖最近引起了相当大的关注。基质辅助激光解吸/电离质谱（MALDI-MS）在分析生物分子方面非常强大，而其在聚糖中的应用仍然具有挑战性。在这里，一种新型的反应性基质辅助激光解吸/电离(MALDI)基质，2-肼基对苯二甲酸，是合理设计和合成的。它提供了与聚糖的均匀共结晶，并且仅在负离子模式下产生具有高强度的去质子化离子。与芥子酸结合使用，本研究建立了一种快速,高通量的聚糖分析方法,该方法在飞摩尔水平具有较高的检测限,且线性良好(R2>0.999).此外，所建立的方法已成功地用于定量桃的不同品种和组织中的N-聚糖[Prunuspersica（L.）巴奇]。我们的工作表明N-聚糖作为食源性过敏的生物标志物的潜在作用，并为阐明碳水化合物表位与食物过敏之间的可能关系奠定了方法学基础。
Glycans recently attracted considerable attention as the proposal of cross-reactive carbohydrate determinants for food allergy. Matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) is powerful in analyzing biomolecules, while its applications in glycans are still challenging. Herein, a novel reactive matrix-assisted laser desorption/ionization (MALDI) matrix, 2-hydrazinoterephthalic acid, was rationally designed and synthesized. It provides uniform co-crystallization with glycans and only produces deprotonated ions with high intensities in the negative-ion mode. In combination with sinapic acid, a rapid and high-throughput method was established for on-target analysis of glycans with a superior limit of detection at the femtomole level and a good linearity (R2 > 0.999). Furthermore, the established method was successfully applied to quantify N-glycans in different cultivars and tissues of peach [Prunus persica (L.) Batsch]. Our work suggests the potential role of N-glycans as biomarkers for food-borne allergy and lays a methodological foundation for the elucidation of the possible relationship between carbohydrate epitopes and food allergy.
