设计并合成了具有长链烷基和苄基结构的磁性离子液体(MIL)苄基三辛基铵硫氰酸钴(II)[N8,8,8,B+]2[Co(SCN)42-]作为微萃取相。设计的新结构MIL显示良好的疏水性,对芳香族和脂肪族化合物的高提取能力,并具有明显的颜色标记(蓝色),易于通过磁铁从水溶液中视觉分离。在目前的工作中,一个绿色的,基于同时衍生和萃取芳烃(酪胺,组胺,苯乙胺,进行了色胺)和脂肪族(亚精胺和精胺)生物胺(BA)。建立了微波辅助衍生-MIL分散液液微萃取(DLLME)高效液相色谱法测定不同食品样品中6种BAs的方法。该方法已成功应用于啤酒和牛奶样品的分析。分析物的回收率分别为93.0-110.3%和91.2-111.6%,分别。六个BA的检出限(LOD)为0.51-1.49μgL-1。这些结果表明,所提出的方法提供了一种有效的,准确,以及食品样品中BAs残留检测的灵敏方法,该方法对于大量样品的常规分析测量不同种类的化合物具有很大的潜力。
A multifunctional visual observation of magnetic ionic liquid (MIL) benzyltrioctylammonium thiocyanatecobalt (II) [N8,8,8,B+]2[Co(SCN)42-] with the long-chain alkyl and benzyl group structures was designed and synthesized as microextraction phase. Designed new structural MIL displays good hydrophobicity, high extraction capacity for both aromatic and aliphatic compounds, and has obvious color markers (blue color) which is easy visual separation from aqueous solution through a magnet. In the present work, a green, efficient and rapid samples pretreatment method based on simultaneous derivatization and extraction of aromatic (tyramine, histamine, phenylethylamine, tryptamine) and aliphatic (spermidine and spermine) biogenic amines (BAs) were performed. Microwave-assisted derivatization coupled with MIL-dispersive liquid-liquid microextraction (DLLME) was established for the determination of six BAs in different food samples via HPLC. The method was successfully applied for the analysis of beer and milk samples, and the recoveries of analytes were 93.0-110.3% and 91.2-111.6%, respectively. The limits of detection (LODs) were 0.51-1.49 μg L-1 for six BAs. These results have demonstrated that the proposed method has offered an effective, accurate, and sensitive methodology for BAs residue detection in food sample, and this method has great potential for the routine analysis of large numbers of samples on measuring different kinds of compounds.