双酚AF(BPAF),作为BPA的结构类似物之一,近年来越来越多地使用。然而,有限的研究表明其不良反应与BPA相似或高于BPA.为了探讨BPAF亚急性暴露的一般毒性和遗传毒性,应用新型28天多终点(Pig-a测定+微核[MN]试验+彗星测定)基因毒性评价平台.雄性大鼠随机分为7个主要实验组和4个卫星组。主要实验组包括BPAF治疗组(0.5、5和50μg/kg·bw/d),BPA组(10μg/kg·bw/d),两个溶剂对照组(PBS和0.1%乙醇/99.9%油),和一个阳性对照组(N-乙基-N-亚硝基脲,40mg/kgbw)。卫星组包括BPAF高剂量恢复组(BPAF-HR),采油组(油-R),ENU恢复组(ENU-R),和PBS恢复组(PBS-R)。所有组均通过管饲法连续28天口服。和卫星组被给予35天的恢复期。在所有组织病理学检查的器官中,注意到睾丸和附睾损伤,这进一步表现为血-睾丸屏障(BTB)连接蛋白(连接蛋白43和Occludin)的破坏。BPAF可以诱导微核产生和DNA损伤,但是基因毒性损伤可以在恢复期后修复。BPAF下调DNA修复基因OGG1的表达。总结一下,在这个实验的设计下,注意到BPAF的雄性生殖毒性,类似于双酚A,但其诱导微核产生的能力可能强于BPA。
Bisphenol AF (BPAF), as one of structural analogs of BPA, has been increasingly used in recent years. However, limited studies have suggested its adverse effects similar to or higher than BPA. In order to explore the general toxicity and genotoxicity of subacute exposure to BPAF, the novel 28-day multi-endpoint (Pig-a assay +
micronucleus [MN] test + comet assay) genotoxicity evaluation platform was applied. Male rats were randomly distributed into seven main experimental groups and four satellite groups. The main experimental groups included BPAF-treated groups (0.5, 5, and 50 μg/kg·bw/d), BPA group (10 μg/kg·bw/d), two solvent control groups (PBS and 0.1% ethanol/99.9% oil), and one positive control group (N-ethyl-N-nitrosourea, 40 mg/kg bw). The satellite groups included BPAF high-dose recovery group (BPAF-HR), oil recovery group (oil-R), ENU recovery group (ENU-R), and PBS recovery group (PBS-R). All groups received the agents orally via gavage for 28 consecutive days, and satellite groups were given a recovery period of 35 days. Among all histopathologically examined organs, testis and epididymis damage was noticed, which was further manifested as blood-testis barrier (BTB) junction protein (Connexin 43 and Occludin) destruction. BPAF can induce
micronucleus production and DNA damage, but the genotoxic injury can be repaired after the recovery period. The expression of DNA repair gene OGG1 was downregulated by BPAF. To summarize, under the design of this experiment, male reproductive toxicity of BPAF was noticed, which is similar to that of BPA, but its ability to induce
micronucleus production may be stronger than that of BPA.