革兰氏阴性,杆状,非运动和严格的需氧菌株,指定NBU2979T,与位于东海梅山岛的沿海滩涂隔离。菌株NBU2979T在32°C时生长最佳,与2.0%NaCl(w/v)和pH7.0-7.5。主要脂肪酸(>10%)是异C15:0。主要的极性脂质包括磷脂酰甘油,磷脂酰乙醇胺,二磷脂酰甘油,磷脂酰二甲基乙醇胺,磷脂酰胆碱,一种身份不明的糖脂,两种身份不明的氨基磷脂,一种身份不明的磷脂和一种身份不明的脂质。唯一的呼吸醌是泛醌-8。16SrRNA基因序列的比较分析表明,菌株NBU2979T与SediminisMarinicellaF2T的相似性最高(98.0%),Marinicella码头S1101T(97.5%),LitoralisMarinicellaKMM3900T(96.6%),Marinicellarhbeformis3539T(95.5%),pacificaMarinicellasw153T(95.2%)和明胶MarinicellaS6413T(94.9%)。系统发育分析表明,菌株NBU2979T与Marinicella属成簇,并与菌株M.sediminisF2T密切相关。菌株NBU2979T与Marinicella属的相关物种之间的平均核苷酸同一性和数字DNA-DNA杂交值远低于原核物种划定的阈值极限。菌株NBU2979T的DNAG+C含量为51.6mol%。基于它的表型,化学分类学和基因型数据,菌株NBU2979T(=KCTC82911T=MCCC1K06402T)被认为是Marinicella属新物种的代表,名字叫梅山Marinicellasp。11月。是提议的。
A Gram-negative, rod-shaped, non-motile and strictly aerobic strain, designated NBU2979T, was isolated from a coastal mudflat located on Meishan Island in the East
China Sea. Strain NBU2979T grew optimally at 32 °C, with 2.0 % NaCl (w/v) and at pH 7.0-7.5. The predominant fatty acid (>10 %) was iso-C15 : 0. The major polar lipids included phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidyldimethylethanolamine, phosphatidylcholine, an unidentified glycolipid, two unidentified aminophospholipids, an unidentified phospholipid and an unidentified lipid. The only respiratory quinone was ubiquinone-8. Comparative analysis of 16S rRNA gene sequences showed that strain NBU2979T exhibited highest similarity to Marinicella sediminis F2T (98.0 %),
Marinicella marina S1101T (97.5 %),
Marinicella litoralis KMM 3900T (96.6 %),
Marinicella rhabdoformis 3539T (95.5 %),
Marinicella pacifica sw153T (95.2 %) and
Marinicella gelatinilytica S6413T (94.9 %). Phylogenetic analyses indicated that strain NBU2979T clustered with the genus
Marinicella and was closely related to strain M. sediminis F2T. The average nucleotide identity and digital DNA-DNA hybridization values between strain NBU2979T and related species of genus Marinicella were well below the threshold limit for prokaryotic species delineation. The DNA G+C content of strain NBU2979T was 51.6 mol%. Based on its phenotypic, chemotaxonomic and genotypic data, strain NBU2979T (=KCTC 82911T=MCCC 1K06402T) is considered to be a representative of a novel species in the genus Marinicella, for which the name
Marinicella meishanensis sp. nov. is proposed.