Abstract：
A Gram-negative, rod-shaped, non-motile and strictly aerobic strain, designated NBU2979T, was isolated from a coastal mudflat located on Meishan Island in the East China Sea. Strain NBU2979T grew optimally at 32 °C, with 2.0 % NaCl (w/v) and at pH 7.0-7.5. The predominant fatty acid (>10 %) was iso-C15 : 0. The major polar lipids included phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidyldimethylethanolamine, phosphatidylcholine, an unidentified glycolipid, two unidentified aminophospholipids, an unidentified phospholipid and an unidentified lipid. The only respiratory quinone was ubiquinone-8. Comparative analysis of 16S rRNA gene sequences showed that strain NBU2979T exhibited highest similarity to Marinicella sediminis F2T (98.0 %), Marinicella marina S1101T (97.5 %), Marinicella litoralis KMM 3900T (96.6 %), Marinicella rhabdoformis 3539T (95.5 %), Marinicella pacifica sw153T (95.2 %) and Marinicella gelatinilytica S6413T (94.9 %). Phylogenetic analyses indicated that strain NBU2979T clustered with the genus Marinicella and was closely related to strain M. sediminis F2T. The average nucleotide identity and digital DNA-DNA hybridization values between strain NBU2979T and related species of genus Marinicella were well below the threshold limit for prokaryotic species delineation. The DNA G+C content of strain NBU2979T was 51.6 mol%. Based on its phenotypic, chemotaxonomic and genotypic data, strain NBU2979T (=KCTC 82911T=MCCC 1K06402T) is considered to be a representative of a novel species in the genus Marinicella, for which the name Marinicella meishanensis sp. nov. is proposed.
摘要：
革兰氏阴性，杆状,非运动和严格的需氧菌株，指定NBU2979T,与位于东海梅山岛的沿海滩涂隔离。菌株NBU2979T在32°C时生长最佳，与2.0％NaCl（w/v）和pH7.0-7.5。主要脂肪酸(>10%)是异C15:0。主要的极性脂质包括磷脂酰甘油，磷脂酰乙醇胺，二磷脂酰甘油，磷脂酰二甲基乙醇胺，磷脂酰胆碱，一种身份不明的糖脂,两种身份不明的氨基磷脂，一种身份不明的磷脂和一种身份不明的脂质。唯一的呼吸醌是泛醌-8。16SrRNA基因序列的比较分析表明，菌株NBU2979T与SediminisMarinicellaF2T的相似性最高（98.0％），Marinicella码头S1101T（97.5%），LitoralisMarinicellaKMM3900T（96.6%），Marinicellarhbeformis3539T（95.5％），pacificaMarinicellasw153T（95.2%）和明胶MarinicellaS6413T（94.9%）。系统发育分析表明，菌株NBU2979T与Marinicella属成簇，并与菌株M.sediminisF2T密切相关。菌株NBU2979T与Marinicella属的相关物种之间的平均核苷酸同一性和数字DNA-DNA杂交值远低于原核物种划定的阈值极限。菌株NBU2979T的DNAG+C含量为51.6mol%。基于它的表型，化学分类学和基因型数据，菌株NBU2979T（=KCTC82911T=MCCC1K06402T）被认为是Marinicella属新物种的代表，名字叫梅山Marinicellasp。11月。是提议的。
