■目前,缺乏客观的定量措施来全面评估axSpA的炎症活性,这对准确评估疾病活动提出了一定的挑战。
■探讨骶髂关节(SIJs)MRI弛豫测量和外周血黏膜相关不变T(MAIT)细胞联合参数模型在评估轴性脊柱关节炎(axSpA)炎症活动中的价值。
■这项回顾性临床研究包括88名axSpA患者(中位年龄31.0(22.0,41.8)岁,21.6%的女性)和20个对照组(中位年龄28.0(20.5,49.5)岁,40.0%女性)。根据ASDAS-CRP将axSpA组分为有活性亚组(n=50)和无活性亚组(n=38)。所有参与者均接受SIJsMRI检查,包括T1和T2*标测,和MAIT细胞(定义为CD3+Vα7.2+CD161+)及其活化标志物(CD69)的外周血流式细胞术分析。T1和T2*值,比较不同组之间MAIT细胞和CD69+MAIT细胞的百分比。使用逻辑回归建立组合参数模型,采用ROC曲线评价诊断效能。
■axSpA组及其亚组SIJs和%CD69MAIT细胞的T1值均高于对照组(p<0.05),而MAIT细胞%均低于对照组(p<0.05)。T1值与%CD69+MAIT细胞呈正相关,而%MAIT细胞负相关,与ASDAS-CRP(r=0.555,0.524,-0.357,p<0.001)。在对照组和axSpA组之间,在不活跃和活跃的亚组之间,联合参数模型T1映射+%CD69+MAIT细胞具有最佳疗效(AUC=0.959,0.879,灵敏度=88.6,70%,特异性=95.0,94.7%,分别)。
■组合参数模型T1作图%CD69MAIT细胞可以更准确地评估炎症活性水平。
UNASSIGNED: Currently, there is a lack of an objective quantitative measure to comprehensively evaluate the inflammatory activity of axSpA, which poses certain challenges in accurately assessing the disease activity.
UNASSIGNED: To explore the value of combined-parameter models of sacroiliac joints (SIJs) MRI relaxometry and peripheral blood Mucosal-associated invariant T (MAIT) cells in evaluating the inflammatory activity of axial spondyloarthritis (axSpA).
UNASSIGNED: This retrospective clinical study included 88 axSpA patients (median age 31.0 (22.0, 41.8) years, 21.6% females) and 20 controls (median age 28.0 (20.5, 49.5) years, 40.0% females). The axSpA group was classified into active subgroup (n=50) and inactive subgroup (n=38) based on ASDAS-CRP. All participants underwent SIJs MRI examination including T1 and T2* mapping, and peripheral blood flow cytometry analysis of MAIT cells (defined as CD3+Vα7.2+CD161+) and their activation markers (CD69). The T1 and T2* values, as were the percentages of MAIT cells and CD69+MAIT cells were compared between different groups. Combined-parameter models were established using logistic regression, and ROC curves were employed to evaluate the diagnostic efficacy.
UNASSIGNED: The T1 values of SIJs and %CD69+MAIT cells in the axSpA group and its subgroup were higher than the control group (p<0.05), while %MAIT cells were lower than the control group (p<0.05). The T1 values and %CD69+MAIT cells correlated positively, while %MAIT cells correlated negatively, with the ASDAS-CRP (r=0.555, 0.524, -0.357, p<0.001). Between the control and axSpA groups, and between the inactive and active subgroups, the combined-parameter model T1 mapping+%CD69+MAIT cells has the best efficacy (AUC=0.959, 0.879, sensibility=88.6, 70%, specificity=95.0, 94.7%, respectively).
UNASSIGNED: The combined-parameter model T1 mapping+%CD69+MAIT cells allows a more accurate evaluation of the level of inflammatory activity.