背景:寄生原生动物十二指肠贾第鞭毛虫是人类和动物腹泻病的重要原因,可通过水和环境通过粪便-口腔传播传播,对公共卫生和畜牧业构成挑战。它对中国大型养羊场的影响知之甚少。在这项研究中,我们调查了中国两个地区的大型养羊场中绵羊的十二指肠牛感染和环境污染。河南和宁夏。
方法:共528个粪便样本,从7个大型养羊场采集了402份环境样品和30份水样,从12个后院农场收集了88个粪便样本和13个环境样本。通过靶向β-giardin(bg)基因检测到十二指肠G的存在,通过分析三个基因:bg,谷氨酸脱氢酶(gdh)和三磷酸异构酶(tpi)。
结果:总的十二指肠氏杆菌检出率为7.8%,粪便中1.4%和23.3%,环境和水样,分别。在测试的大型养羊场上,发现河南绵羊的感染率(13.8%)明显高于宁夏绵羊的感染率(4.2%)(P<0.05)。然而,河南(1.9%)和宁夏(1.0%)的环境污染率差异不显著(P>0.05)。对不同生理阶段绵羊的调查表明,妊娠晚期母羊的感染率最低(1.7%),幼羊的感染率最高(18.8%)。遗传分析确定了属于两个组合的十二指肠葡萄球菌,A和E,组合E占主导地位。共鉴定出27种多基因座基因型的E.组合成员
结论:结果表明,在河南和宁夏的大规模养羊场,中国,并且存在环境污染的风险。这项研究是对中国大型养羊场中十二指肠G的存在进行的首次全面检查。需要积极应对十二指肠牛对绵羊养殖场的挑战,以确保公共卫生安全。
BACKGROUND: The parasitic protozoan Giardia duodenalis is an important cause of diarrheal disease in humans and animals that can be spread by fecal-oral transmission through water and the environment, posing a challenge to public health and animal husbandry. Little is known about its impact on large-scale sheep farms in
China. In this study we investigated G. duodenalis infection of sheep and contamination of the environment in large-scale sheep farms in two regions of
China, Henan and Ningxia.
METHODS: A total of 528 fecal samples, 402 environmental samples and 30 water samples were collected from seven large-scale sheep farms, and 88 fecal samples and 13 environmental samples were collected from 12 backyard farms. The presence of G. duodenalis was detected by targeting the β-giardin (bg) gene, and the assemblage and multilocus genotype of G. duodenalis were investigated by analyzing three genes: bg, glutamate dehydrogenase (gdh) and triphosphate isomerase (tpi).
RESULTS: The overall G. duodenalis detection rate was 7.8%, 1.4% and 23.3% in fecal, environmental and water samples, respectively. On the large-scale sheep farms tested, the infection rate of sheep in Henan (13.8%) was found to be significantly higher than that of sheep in Ningxia (4.2%) (P < 0.05). However, the difference between the rates of environmental pollution in Henan (1.9%) and Ningxia (1.0%) was not significant (P > 0.05). Investigations of sheep at different physiological stages revealed that late pregnancy ewes showed the lowest infection rate (1.7%) and that young lambs exhibited the highest (18.8%). Genetic analysis identified G. duodenalis belonging to two assemblages, A and E, with assemblage E being dominant. A total of 27 multilocus genotypes were identified for members of assemblage E.
CONCLUSIONS: The results suggest that G. duodenalis is prevalent on large-scale sheep farms in Henan and Ningxia,
China, and that there is a risk of environmental contamination. This study is the first comprehensive examination of the presence of G. duodenalis on large-scale sheep farms in
China. Challenges posed by G. duodenalis to sheep farms need to be addressed proactively to ensure public health safety.